5-Aza-2’-deoxycytidine and 4-phenylbutyric acid exert a cooperative effect on the expression of miR-196b in chronic myeloid leukemia cells

doi:10.3969/j.issn.0529-1356.2014.04.014

Abstract

Abstract:

Objective To study if 5-Aza-2’-deoxycytidine along or together with 4-phenylbutyric acid could affect miR-196b expression levels in chronic myeloid leukemia cells.
Methods K562 cells were treated with DNA methylation inhibitor 5-Aza-2’-deoxycytidine, histone deacetylase inhibitors 4-phenylbutyric acid separately and the combined treatment with both of them, then expression levels of miR-196b were detected using Real-time PCR. Results The half inhibition concentration of 4-phenylbutyric acid was 1.58mmol/L. Comparing with the expression level of miR-196b in normal human bone marrow cells, the expression levels of miR-196b were significantly lower in Aza group, PBA group and negative control cells and nearly consistent among three groups, and as high as normal cells in combined treatment group. Conclusion The expression level of miR-196b in K562 cells could not return to normal treated with 5-Aza-2’-deoxycytidine or 4-phenylbutyric acid separately, while could restore normal when treated with both agents, indicating that miR-196b expression level in K562 cells is related with both DNA methylation and histone acetylation.

Key words: miR-196b, Methylation, Histone acetylation, K562cell, Real-time PCR, Human

LIU Yue SHUAI Chun LI Jie-sheng YIN Hong SONG Yan-bin MA Wen-li*. 5-Aza-2’-deoxycytidine and 4-phenylbutyric acid exert a cooperative effect on the expression of miR-196b in chronic myeloid leukemia cells[J]. AAS, 2014, 45(4): 521-524.

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