Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells

doi:10.3969/j.issn.0529-1356.2014.04.026

Abstract

Abstract:

Objective To study the application of Cell Counting Kit-8(CCK-8) in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cell.
Methods The proliferation of K562 cells was detected by CCK-8 with different concentrations of 5-Aza-2’-deoxycytidine and the cell cycle and apoptosis of K562 cells were detected after K562 treated by 50% inhibitory concentration of 5-Aza-2’-deoxycytidine. Results The 50% inhibitory concentration of 5-Aza-2’-deoxycytidine was 15.55nmol/L, after treated with this concentration, K562 cells showed that G2 phase arrest occurred, proliferation inhibited and apoptosis peaks appeared. Conclusion Inhibition of proliferation of K562 cells with different concentrations of 5-Aza-2’-deoxycytidine varied in a dose-dependent relationship, and 5-Aza-2’-deoxycytidine could promote apoptosis of K562 cells. CCK-8 can be used in detecting the effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells.

Key words: Cell Counting Kit-8, 5-Aza-2’-deoxycytidine, K562, Proliferation

LIU Yue SHUAI Chun LI Jie-sheng YIN Hong SONG Yan-bin MA Wen-li*. Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells[J]. AAS, 2014, 45(4): 582-584.

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