Long-term evaluation of functional recovery and nerve regeneration following tubulation repair of nerve defects in mice

doi:10.3969/j.issn.0529-1356.2014.05.003

Abstract

Abstract:

Objective This study is to identify long-term functional recovery and maturity of regenerated nerve fibers after repairing mouse nerve defects with chitosan/polylactide-co-polyglycolide artificial nerve grafts (CPANGs). Methods Mouse sciatic nerve defects, 2mm in length, were bridged by CPANGs (n=6), with nerve autograft (n=6) and nerve defect (n=6) as controls. Plantar test, electrophysiological examination and laser Doppler perfusion imaging following nerve crush were carried out at 1 year after repair to assess nerve function recovery, while muscle wet weight ratio, histological assessment and transmission electron microscopy were performed to evaluate nerve re-innervation and maturity of regenerated nerve fibers. Results When compared to the autograft group, the CPANG group did not show statistically significant difference in functional recovery in terms of paw withdrawal latency, neurogenic vasodilatation, amplitude and latency of compound muscle action potentials (CMAPs), wet weight ratio of gastrocnemius and tibialis cranialis muscles, number of myelinated nerve fibers and density of unmyelinated axons. However, both these two repair groups exhibited significantly longer CMAPs latency, thinner myelin sheath and a lagbehind shift of diameter distribution of myelinated axons as compared to the normal control. Conclusion At 1 year after the mouse sciatic nerve defect was repaired by CPANGs, sensory and autonomic nerve function, number of regenerated axons and muscle re-nnervation degree were recovered to the same extent as nerve autografting, but the regenerated nerve fibers were in a state of immaturity.

Key words: Nerve injury, Nerve repair, Chitosan, Polylactide-co-polyglycolide, Lacer doppler perfusion imaging, Electrophysiological examinations, Mouse

MI Da-guo ZHANG Yan-ping GU Tian-wen ZHAO Ya-hong HU Wen*. Long-term evaluation of functional recovery and nerve regeneration following tubulation repair of nerve defects in mice[J]. AAS, 2014, 45(5): 599-604.

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