Effects of a Chinese medicine compound on histological structure and antioxidant enzyme activity in the liver of filial mice injured by ephedrine

doi:10.16098/j.issn.0529-1356.2015.02.018

Abstract

Abstract:

Objective To investigate the effect of a Chinese medicine compound on histological damnification of ephedrine, and understand an important significance for forfend freak-out and safeguard social stability. Methods The filial mice were intraperitoneal injected escalation doses (2.0 g/L，3.0 g/L，4.0 g/L ) of ephedrine，and irrigated to stomach with escalation doses（20.0 g/L，30.0 g/L and 40.0 g/L）of Chinese medicine compounds after injected ephedrine 1 hour later.The samples were withdrawn at 5，10 and 15 days respectively，the activities of superoxide dismutase(SOD)，catalase(CAT)，as well as content of malondialdehyde(MDA) were determined by colorimetry and the liver structures of filial mice were observed by optical microscopy，the expression changes of c-Fos protein were measured by immunohistochemical staining. Results The activity of SOD，CAT of ephedrine groups was lower than that of the control groups in 10 days，15 days，while the content of MDA was higher than control groups (P<0.05 or P<0.01). The filial mice’s liver histology appeared various degrees of damage, and the expression intensity of c-Fos protein in liver of the ephedrine groups was higher than that of the the control groups (P<0.05 or P<0.01).The activity of SOD，CAT of the Chinese medicine groups increased，while the content of MDA decreased (P<0.05 or P<0.01).The liver damage of filial mice of Chinese medicine groups reduced，the expression of c-Fos protein intensity decreased (P<0.05 or P<0.01). Conclusion Ephedrine affects the histological structure and antioxidant enzymes activity of the liver tissue.The Chinese medicine compound may enhance cells antioxidant enzymes activity, and reduce liver tissue damage by injecting ephedrine.

Key words: Chinese medicine compound, Ephedrine, Liver, Enzyme activity, c-Fos protein, Spectrophotometer, Filial mouse

LI Chong-yang YU Shi-yuan*. Effects of a Chinese medicine compound on histological structure and antioxidant enzyme activity in the liver of filial mice injured by ephedrine[J]. AAS, 2015, 46(2): 251-256.

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