Expression of imprinting gene in mouse parthenogenetic embryos and parthenogenetic embryonic stem cells

doi:10.16098/j.issn.0529-1356.2015.05.024

Abstract

Abstract:

The development of mice parthenogenetic embryos can not be beyond 10.5days, because of the defect in extra-embryonic tissues and the abnormal expression of imprinting gene. Establishment of mice diploid or haploid parthenogenetic embryonic stem cells may be applied for searching novel imprinting genes and for cell therapy, which broaden the field of scientific research and regenerative medicine. Aggregation of parthenogenetic embryos as a convenient method not only can notably increase the efficiency of establishing parthenogenetic stem cells but also improve the expression level of imprinting genes. This improvement probably achieves through compensation of imprinting genes expression among different parthenogenetic embryos. In this paper we discuss the expression of imprinting genes in mice parthenogenetic embryos, aggregated parthenogenetic embryos, parthenogenetic haploid embryonic stem cells, parthenogenetic diploid embryonic stem cells and parthenogenetic embryonic stem cells derived from aggregated embryos.

Key words: parthenogenetic stem Parthenogenetic embryonic stem cell, Imprinting gene, Aggregation, Mouse

SONG Si-hang ZHANG Zi-hui LIAO Chen LEI Lei*. Expression of imprinting gene in mouse parthenogenetic embryos and parthenogenetic embryonic stem cells[J]. AAS, 2015, 46(5): 710-714.

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