Influence of vitamin D3 on embryo loss rate and peripheral immune cells in mouse model of spontaneous abortion

doi:10.16098/j.issn.0529-1356.2017.05.017

Abstract

Abstract:

Objective To explore the influence of vitamin D3 (VitD₃) on embryo loss rate and peripheral immune cells in a mouse model of spontaneous abortion. Methods CBA/J×BALB/c was adopted to establish the mouse model of normal pregnancy, while CBA/J×DBA/2 was adopted to establish the mouse model of spontaneous abortion. There were totally 5 groups: normal group, spontaneous abortion group, VitD₃ low dose group, middle dose group and high dose groups. The VitD₃ groups were treated by VitD₃ solution diluted in saline with intraperitoneal injection of 1μg, 4μg, 16μg, respectively, 15 cases per group. The embryo loss situation in each group was recorded and observed. The regulatory T cells (Treg) factor, interleukin-10 (IL-10) and helper T cells 17(Th17) cytokines, interleukin-17A (IL-17A), as well as the Treg and Th17 cell were detected. Results Compared with the normal group, a significant rise of embryo loss rate presented in spontaneous abortion group (χ2=16.045, P<0.05); Compared with spontaneous abortion group, the embryo loss rate in VitD₃low dose group, middle dose group and high dose group reduced significantly (χ2=5.881, 13.704, 15.663, P<0.05). Compared with the normal group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in spontaneous abortion group reduced significantly; Th17 and IL-17A increased significantly, and the differences were statistically significant (P<0.05). Compared with spontaneous abortion group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in VitD₃low dose group, middle dose group and high dose group increased significantly, while the Th17 and IL-17A decreased significantly, and they all had significantly difference based on statistical analysis (P<0.05).Conclusion VitD₃can reduce the embryo loss rate in the mouse model of spontaneous abortion, which may be related to adjusting the balance of Th17/Treg.

Key words: Vitamin D3, Spontaneous abortion, Embryo loss, Regulatory T cell/Helper T cell 17, Flow cytometry, Mouse

XU Hui WANG Yu-lan SONG Xiao-hui HUANG Juan. Influence of vitamin D3 on embryo loss rate and peripheral immune cells in mouse model of spontaneous abortion[J]. Acta Anatomica Sinica, 2017, 48(5): 595-599.

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