Optimization of mouse in utero electroporation technique

doi:10.16098/j.issn.0529-1356.2018.03.020

Abstract

Abstract:

Objective To optimize the in utero electroporation protocol for transfering genes into the embryonic murine brain. Methods Critical steps of in utero electroporation were optimized, including choosing isoflurane anesthesia, improving the preparation of micropipettes, reducing the exposure time of embryos during the surgery as well as optimizing electroporation parameters. Forty-two mouse embryos from 6 mothers were subjected to in utero electroporation at gestational day 16.5 using the optimized protocol. Results The survival rates of mothers and embryos were 100%. The tranfered gene was expressed in 85%±3% of electroporated embryonic brains. Conclusion Using the optimized in utero electroporation protocol, a satisfying survival rate of embryos and transfection efciency can be achieved.

Key words: Brain, Gene, Development, In utero electroporation, Mouse

YANG Xiao-wei LIU An-jun REN An-jing WANG Zhi-BinZHANG Wei-ping Lü Jian-xin XIE Zhi-fang. Optimization of mouse in utero electroporation technique[J]. Acta Anatomica Sinica, 2018, 49(3): 391-394.

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