Changes on dopamin D1 receptor expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy#br#

doi:10.16098/j.issn.0529-1356.2020.04.003

Abstract

Abstract:

Objective To observe the changes of dopamin D₁ receptor (D₁DR) expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy. Methods Thirty pregnant rats were randomly divided into three groups namely normal control group, low-dose radiation group and high-dose radiation group.Eighty newborn rats were randomly kept in each group. The offspring rats’prefrontal cortices were collected at day 1, day 10, day 40 and day 60 after birth. The position and intensity of D₁DR were detected by immunohistochemistry, and the relative expression of D₁DR mRNA was detected by Real-time PCR. Results The experimental result showed that D₁DR was expressed in the cytoplasm, membrane and axon of neurons. The expression intensity of D₁DR of the two radiation groups was significantly lower than that of the normal control group(P<0.05) and the expression intensity on D₁DR of the high-dose radiation group was significantly lower than that of the low-dose radiation group(P<0.05). The result of Real-time PCR was consistent with those of immunohistochemistry. Conclusion Exposure to mobile phone radiation during pregnancy could result decreased D1DR expression level in the prefrontal cortex of offspring rats.

Key words: Pregnancy, Mobilephone tradition, Dopamin D1 receptor, Real-time PCR, Offspring rat

CLC Number:

R7I5.3

LI Rong WANG Ling-xing LIU Liang-cai ZHANG Yan-qin. Changes on dopamin D1 receptor expression in the prefrontal cortex of offspring rats exposed to mobilephone radiation during pregnancy#br#[J]. Acta Anatomica Sinica, 2020, 51(4): 491-494.

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