Objective  To observe the effect of electroacupuncture on the expression of spinal prostatic acid phosphatase (PAP)  mRNA and adenosine concentration in neuropathic pain rats.   Methods Twenty-one male adult SD rats were randomized into sham operation group(sham group),chronic constrictive injury group(CCI group) and electroacupuncture treatment group(EA group). Electro acupuncture treatment was applied to Tsusanli(ST36) and Taichung(LR3) points.The mechanical pain threshold and thermal pain threshold were measured by using electronic von frey anesthesiometer and rediant-heat irradiation separately on pre-CCI 1 day, post-CCI 7 days and post-EA treatment. Real-time PCR was applied to measure the expression of spinal cord PAP mRNA. High performance liquid chromatography(HPLC) was applied to measure spinal cord adenosine concentration.   Results Compared with the CCI group rats,mechanical and thermal pain thresholds in EA group rats were increased significantly(P＜0.05, P＜0.05). The expression of PAP mRNA in the spinal cord in EA group rats was increased significantly compared with  the CCI group rats(P＜0.05).Simultaneously，the extracellular concentration of spinal adenosine in EA group rats was increased compared with the CCI group rats(P<0.01).   Conclusion  It demonstrated electroacupuncture can improve mechanical and thermal pain thresholds in neuropathic pain rats, and PAP and adenosine may be involved into the analgesia mechanism in spinal cord.