Objective  To investigate the effect of wingless-type MMTV integration site family member 5b(Wnt5b) gene overexpression mediated by recombinant adenovirus on the differentiation of mouse embryonic liver stem cells and repair of chronic liver injury in mice.    Methods  Recombinant adenoviruses expressing Wnt5b and green fluorescent protein (GFP) were applied respectively to infect mouse fetal liver stem cells HP14-19, and induced its differentiation and verified the expression of Wnt5b through Real-time PCR and Western blotting. It also applied indocyanine grean(ICG) uptake experiment and periodic acid-schiff(PAS) staining to detect the differentiation ability of HP14-19 into hepatocyte-like cells. The Real-time PCR was chosen to detect hepatocyte markers albumin (Alb) and cytokeratin 18 (CK18) expression. Forty-eight experimental male BALB/c mice were randomly divided into control group, model group, stem cell treatment group and Wnt5b modified stem cell treatment group. The carbon tetrachloride(CCl4) was selected to establish a chronic liver injury model. After 2 weeks of stem cell treatment, liver pathological staining and immunohistochemistry were used to evaluate liver repair.  Results  Both Real-time PCR and Western blotting detection showed that Wnt5b was successfully overexpressed in HP14-19（P<0.001）. The mRNA expressions of Alb and CK18 in the Wnt5b group were higher than those in the GFP group and control group by Real-time PCR（P<0.001）. ICG uptake test and PAS staining showed that the ICG uptake capacity and glycogen synthesis capacity of Wnt5b group increased significantly compared with the GFP group after 7 days of induction（P<0.01）. Liver HE staining result  showed that the model group had an obvious liver injury and liver tissue structure disordered. The stem cell group liver tissue structure partially recovered, and the liver tissue recovery in the Wnt5b group was better than that in the stem cell group. Masson staining showed that the model group had obvious fiber hyperplasia, and the Wnt5b group had less blue-stained fiber than the stem cell group（P<0.05）. The immunohistochemistry showed collagen type Ⅰ(ColⅠ) and myeloperoxidase (MPO) increased significantly in the  model group, and the repair effects of these indexes were significant in the Wnt5b group than in the fetal stem cell group（P<0.05）.    Conclusion  Wnt5b can induce mouse fetal liver stem cells to differentiate into mature hepatocyte-like cells. Also, the genetically modified fetal liver stem cells have more advantages in repairing the liver of chronic liver injury mice than fetal liver stem cells.