Morphological changes and expression of nerve growth factor of hippocampus in different ages of Bactrian camels

doi:10.16098/j.issn.0529-1356.2015.04.002

Abstract

Abstract:

Objective To investigate morphological changes of neurons and the expression of nerve growth factor (NGF) in different areas of hippocampus in fetal, young and adult Bactrian camels. Methods Nissl staining was used to observe morphological changes of pyramidal cells in CA1-CA3 areas and granule cells of dentate gyrus. Immunohistochemical method was used to detect the expression of NGF of hippocampus in fetal, young and adult Bactrian camels. Results The results of Nissl staining showed that from fetal Bactrian camel to young Bactrian camel and to adult Bactrian camel, pyramidal cells in CA1-CA3 areas and granular cells in teh dentate gyrus enlarged in volume but decreased gradually in density with the growth of Bactrian camel. The proportion of nuclei in neurons also decreased. Immunohistochemical analysis revealed that the expression level of NGF in pyramidal cells of adult camel was significantly higher than the other two periods of Bactrian camel (P<0.01). Conversely, the expression level of NGF in granule cells increased consistently from fetal camel to adult camel. Conclusion From fetal Bactrian camel to adult Bactrian camel, the main neurons of hippocampus continuously become mature: neurons change from small and global cells which have little cytoplasm, to neurons which are large and have specific morphology and function. The high expression level of NGF in hippocampus of adult camel indicates that NGF could regulate differentiated neurons, propel morphological and functional changes in neurons, which will consequently contribute to the improvement of learning and memory functions of hippocampus in Bactrian camel.

Key words: Hippocampus, Development, Pyramidal cells, Granule cells, Nerve growth factor, Bactrian camel

ZHANG Yuan XIE Li WANG Chang-jiang YUE Ming-xia WANG De-gui SHAO Bao-ping WANG Jian-lin*. Morphological changes and expression of nerve growth factor of hippocampus in different ages of Bactrian camels[J]. AAS, 2015, 46(4): 443-449.

References

［1］Andersen P, Morris R, Amaral D, et al. The Hippocampus Book［M］. New York: Oxford University Press, Inc. 2007: 22-24.
［2］Guo WCh, Zhang JB, Chen ShN. Breeds, breeding, feeding, management and utilization of Bactrian camel in China.(2)［J］. Special Economic Animal and Plant, 2014, 17(7): 4-8.（in Chinese）
郭文场, 张嘉保, 陈树宁. 中国双峰驼的品种, 繁殖, 饲养管理和利用(2)［J］. 特种经济动植物, 2014, 17(7): 4-8.
［3］Chen J, Bai Z, Gao C, et al. Morphology of rhinencephalon and hippocampal formation of the Bactrian camel (camelus bactrianus) with their adaptive features［J］. Vet Res Commun, 2009, 33(1): 25-32.
［4］Yang QJ, Dou ZhH, Li ZhX, et al. Study in neuron apoptosis in CA3 area during the development of hippocampus in rat［J］. China Journal of Gerontology, 2003, 23(8): 530-532.（in Chinese）
杨秋佳, 窦肇华, 李质馨, 等. 大鼠海马CA3区发育中神经细胞凋亡的研究［J］. 中国老年学杂志, 2003, 23(8): 530-532. 
［5］Kim WR, Sun W. Programmed cell death during postnatal development of the rodent nervous system［J］. Dev Growth Differ, 2011, 53(2): 225-235.
［6］Janigro D. The Cell Cycle in the Central Nervous System ［M］. Totowa, New Jersey: Humana Press, 2006: 71-79.
［7］Zhang JK, Zhang L, Wang LP, et al. Cell apoptosis during the development and aging of hippocampus in C57/BL6 mice［J］. Journal of Xi’an Jiaotong University(Medical Sciences), 2012, 33(3): 308-311.（in Chinese）
张敬坤, 张莉, 王丽鹏, 等. C57/BL6 小鼠海马结构发生, 发育和衰老过程中的细胞凋亡［J］. 西安交通大学学报（医学版）, 2012, 33(3): 308-311.
［8］KhalafNazzal R, Francis F. Hippocampal development-old and new findings［J］. Neuroscience, 2013, 248: 225-242.
［9］Deng JB, Cai Y, Sun XJ, et al. The morphological study on hippocampal development of human fetuses——Ⅰ.Observation of general structure［J］. Chinese Journal of Neuroanatomy, 1996, 12(1): 1-9.（in Chinese）
邓锦波, 蔡琰, 孙晓江, 等. 人胚胎海马发育的形态学研究——Ⅰ.一般结构观察［J］. 神经解剖学杂志, 1996, 12(1): 1-9.
［10］Encinas JM, Michurina TV, Peunova N, et al. Division-coupled astrocytic differentiation and age-related depletion of neural stem cells in the adult hippocampus［J］. Cell Stem Cell, 2011, 8(5): 566-579.
［11］Curlik DM, Maeng LY, Agarwal PR, et al. Physical skill training increases the number of surviving new cells in the adult hippocampus［J］. PLoS One, 2013, 8(2): e55850.
［12］Maisonpierre PC, Belluscio L, Friedman B, et al. NT-3, BDNF, and NGF in the developing rat nervous system: parallel as well as reciprocal patterns of expression［J］. Neuron, 1990, 5(4): 501-509.
［13］Aloe L, Rocco ML, Bianchi P, et al. Nerve growth factor: from the early discoveries to the potential clinical use［J］. J Transl Med, 2012, 10(239): 23-30.
［14］Aloe L, Rocco ML, Bianchi P, et al. Nerve growth factor: from the early discoveries to the potential clinical use［J］. J Transl Med, 2012, 10(1): 239-239.

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