Morphological study of Reelin regulation on the development of rostral migratory stream in the mouse

doi:10.16098/j.issn.0529-1356.2015.04.001

Abstract

Abstract:

Objective To investigate the differentiation of neural stem cells in the subventridular zone (SVZ) and neural migration along the the rostral migratory stream (RMS) into the olfactory bulb（OB), particularly, Reelin’s effect on the neural differentiation and migration in RMS. Methods Wild type mice (50 cases) and reeler mice (23 cases) from E16 (embryonic day 16) to P90 (postnatal day 90) were used for Nissl staining, immunofluorescent labeling, ink perfusion and ultrastructure observation to visualize neural stem cells, the radial glial cells and vasulature in neocortex and RMS. The development of RMS in two groups was compared. Results In embryonic days and early postnatal period, vascular niches in the SVZ consisted of neurons, glial cells, neural stem cells and vascular vessel which provided a ideal microenvironment for the differentiation and mturation of neural stem cells. After maturation in the vascular niche, the neural stem cells started to migrate tangentially along RMS toward olfactory bulb with the chain-guidable pattern along both glial and vascular scaffold. After entering in OB, the migrating pattern translated into the radial migration, and these cells differentiated into various neuron in olfactory bulb. In general, RMS’s development, neural stem cell differentiation and cell migration in reeler mice were similar to the wild type, howover, the distribution of vasculature in cortex and RMS was disordered, and the migrating cells often concentrated in olfactory bulb densely and scatteredly. Conclusion Niche in subventricular zone is the main sources of neural stem cells, and both vasculature and radial glia and acts as a scaffold for the migration of neural stem cells in RMS. As an important signal for cell migration, Reelin can affect the development of blood vessels and cell migration in RMS. Without Reelin, the cell migration in RMS will be blocked.

Key words: Rostral migratory stream, Reelin, Olfactory bulb, Vessel, Immunofluorescence, Mouse

SHI Shu-qin CUI Zhan-jun YAN Ming-chao MU Xiao-yun DENG Jin-bo* YIN Ming-wei*. Morphological study of Reelin regulation on the development of rostral migratory stream in the mouse[J]. AAS, 2015, 46(4): 433-442.

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