Effects of acute cold exposure on the mouse central nervous system

doi:10.3969/j.issn.0529-1356.2013.05.002

Abstract

Abstract:

Objective The objective of this study is to explore the effects of acute cold exposure to the mouse central nervous system. Methods To establish an acute cold exposure animal model, 40 adult male mice ofP40-50 were housed at 0℃ to -4℃ environment for 20 days. Estrogen receptors were labeled by immunohistochemistry; the dendritic spines of visual cortical pyramidal cells were labeled by DiI diolistic assay; and synaptic ultrastructure was observed by transmission electron microscopy. Results Acute cold exposure induced a decrease in the dendritic spine densities of mouse visual cortical pyramidal cells, along with a decrease in the number of synapse formations. The ultrastructures of some synapses were observed to have cytoplasmic and organellar swellings, as well as a decrease in microtubules. Furthermore, the proliferation of neural cells was significantly inhibited. Conclusion Acute cold exposure may cause structural and functional changes in the mouse central nervous system, possibly due to the direct participation of estrogen and its receptor, G protein-coupled receptor30 (GPR30), in acute cold response.

Key words: Acute cold response, Dendritic spine, Synapse, Visual cortex, Transmission electron miscroscopy, DiI diolistic assay, Mouse

CUI Zhan-jun ZHAO Kai-bing DENG Jie-xin CHENG Wen-jing XU Gao-lei DENG Jin-bo*. Effects of acute cold exposure on the mouse central nervous system[J]. AAS, 2013, 44(5 ): 594-601.

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