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    2013, Volume 44 Issue 5
    06 October 2013
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    Effect of hippocampal microenvironment on cells’ differentiation and polarity
    FU Xing SHI Zhen-yu JIN Hai-xiao WEN Ya-nan FAN Yuan-yuan DENG Jin-bo*
    2013, 44 (5 ):  586-593.  doi: 10.3969/j.issn.0529-1356.2013.05.001
    Abstract ( )  

    Objective Our study aimed to investigate the influence of the hippocampal microenvironment on cell’sdifferentiation and polarization. Methods The hippocampal slices were co-cultured with five types of cells (liver cancer cells, normal liver cells, PC12 cells, normal nerve cells and bone marrow mesenchymal stem cells) with various differentiations. The cell differentiation and polarization were observed. Results Compared with the control, the cells distributed with laminar pattern, mainly concentrated in pyramidal cell layer and granule cell layer, with double ‘C’ shape. However, the cells distributed evenly elsewhere. Under the inducement of hippocampal microenvironment, low-differentiated cells underwent polarization and differentiation, but the well-differentiated cells did not. Conclusion The hippocampal microenvironment may promote the cell polarization and differentiation and inhibit the cells’ proliferation. The production of cell polarization and differentiation depends on the differentiated status and the microenvironment.

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    Effects of acute cold exposure on the mouse central nervous system
    CUI Zhan-jun ZHAO Kai-bing DENG Jie-xin CHENG Wen-jing XU Gao-lei DENG Jin-bo*
    2013, 44 (5 ):  594-601.  doi: 10.3969/j.issn.0529-1356.2013.05.002
    Abstract ( )  

    Objective The objective of this study is to explore the effects of acute cold exposure to the mouse central nervous system. Methods To establish an acute cold exposure animal model, 40 adult male mice ofP40-50 were housed at 0℃ to -4℃ environment for 20 days. Estrogen receptors were labeled by immunohistochemistry; the dendritic spines of visual cortical pyramidal cells were labeled by DiI diolistic assay; and synaptic ultrastructure was observed by transmission electron microscopy. Results Acute cold exposure induced a decrease in the dendritic spine densities of mouse visual cortical pyramidal cells, along with a decrease in the number of synapse formations. The ultrastructures of some synapses were observed to have cytoplasmic and organellar swellings, as well as a decrease in microtubules. Furthermore, the proliferation of neural cells was significantly inhibited. Conclusion Acute cold exposure may cause structural and functional changes in the mouse central nervous system, possibly due to the direct participation of estrogen and its receptor, G protein-coupled receptor30 (GPR30), in acute cold response.

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    Intraperitoneal injection of 2-DG induces Fos expression in the hypothalamus supraoptic nucleus and paraventricular nucleus of rats
    ZHAO Lin JIA Kun-peng JIN Xiao-hang SHI Juan*
    2013, 44 (5 ):  602-606.  doi: 10.3969/j.issn.0529-1356.2013.05.003
    Abstract ( )  

    Objective To study if intraperitoneal injection of 2-deoxy-D-glucose (2-DG) can functionally activate neurons in the hypothalamus supraoptic nucleus (SON) and paraventricular nucleus (PVN) by induction of Fos expressing.
    Methods Twelve healthy male Sprague-Dawley rats were randomly divided into intraperitoneal injection of 2-DG (6 rats), saline control (3 rats) and normal control (3 rats) groups. After the treatment, immunohistochemical staining was employed to observe the expressions of Fos and double labelling of oxytocin (OT) or vasopressin (VP) with Fos in SON and PVN, respectively. The plasmal level of OT and VP was examined by ELISA assay. Results Compared with saline and normal control groups, 2-DG induced specific Fos expression was mainly located in lateral and perifornical areas of hypothalamus. High levels of Fos immunoreactivity were observed in SON and PVN where they double labeled with the specific neuroactive substances OT and VP. The double labeling rates for OT and Fos (the percentage between the number of OT/Fos doublelabeled neurons to that of OT positive neurons) were 87.10% in SON and 90.57% in PVN, significantly higher than that of VP/Fos double labeling rate (68.42% in SON, 76.92% in PVN, P<0.05). In contrast, the plasma level of OT and VP after injection of 2-DG may not show discernible changes to that of control rat. Conclusion Intraperitoneal injection of 2-DG can induce Fos expression in OT and VP neurons in hypothalamic SON and PVN which may be involved in triggering acute stress response induced by 2-DG.

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    Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons
    ZHANG Yang-hui WEI Xiao-fei* SI Jun-qiang MA Ke-tao ZHANG Lu-lu WANG Qin-wen
    2013, 44 (5 ):  607-611.  doi: 10.3969/j.issn.0529-1356.2013.05.004
    Abstract ( )  

    Objective To explore the electrophysiological properties of cultured hippocampal cholecystokinin (CCK) positive (CCK+ ) neurons. Methods The cultured hippocampal CCK+ neurons were labeled by an adenovirus encoding CCK promoter and green fluorescent protein (GFP) and then whole cell patch clamp technique was carried on to explore the electrophysiology characteristic of hippocampal CCK+ neurons. Results The long diameter of CCK+ neurons’cell body was (22.85±0.77)μm, and the short diameter was (16.21±0.42)μm. The membrane electrophysiology parameters of CCK+ neurons were as following.
    Rest membrane potential was(-55.90±1.30)mV. Membrane capacity was (45.77±2.06)pF. Membrane resistance was (711.00±46.69)MΩ. The action potential firing frequency of cultured hippocampal CCK+ neurons was (26.17±3.41)Hz. The spontaneous inhibitory postsynaptic currents frequency of CCK+ neurons was (5.26±0.71)Hz, which was significant higher compared to the frequency of spontaneous excitatory postsynaptic currents [(3.24±0.62)Hz]. Conclusion The electrophysiology characteristics of hippocampal CCK+ neuron may be colsely related to its modulatory effect in central nervous system.

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    Effect of knocking down N-cadherin expression on the tectum neuronal migration and neurofilament expression during chick embryo development
    YANG Ci-qing LI Qiong FU Su-lei LI Han FAN Yi-feng GUO Zhi-kun LIN Jun-tang*
    2013, 44 (5 ):  612-615.  doi: 10.3969/j.issn.0529-1356.2013.05.005
    Abstract ( )  

    Objective To clarify the role and function of the N-cadherin and its impact on the expression of neurofilaments in central nervous system during the chick embryo development. Methods Fresh eggs were cultured in normal condition for three days (E3), and 3-4ml of egg white was removed from egg, then continuing culture to E5. N-cadherin interference plasmids (pGPU6-GFP-neo-N-cadherin-shRNA) was injected into the optic tectum and then handled by electroporationin vivotechnology. Tissues were obtained at E12 and from each group three embryos were collected, fixed with 4%PFA, embeded with OCT, and cut into frozen sections. Fluorescence immunohistochemistry method was used to analyses the different protein expression. Results Expression of N-cadherin was inhibited, which caused neuronal migration disorder. The expression of NF was also suppressed. Conclusion Inhibition of N-cadherin expression can lead to neuronal migration disorder, affecting the expression of NF.

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    Role of microRNA-181b in oxygen-glucose deprivation -induced N2A cell ischemic injury and its regulation on HSPA5 protein levels
    HAN Song PENG Zhi-feng LI Jun-fa*
    2013, 44 (5 ):  616-620.  doi: 10.3969/j.issn.0529-1356.2013.05.006
    Abstract ( )  

    Objective To explore the role of microRNA-181b(miR-181b )in oxygen-glucose deprivation (OGD)-induced N2A cell ischemic injury and its regulation on HSPA5 protein levels. Methods Using N2A cell OGD model to mimic ischemic injuryin vitro, the extent of N2A cell survival rate was assessed by thiazolyl blue tetrazolium bromide (MTT) assay. The heat shock protein A5 (HSPA5 ) levels and the expression levels of miR-181b and HSPA5 mRNA were determined by using Western blotting and Real-time PCR, respectively. Luciferase reporter assay was performed to identify the direct binding of miR-181b with 3’-UTR of HSPA5 mRNA. Results The miR-181b expression level decreased significantly (P<0.05, n=5 per group) in OGD-treated N2A cells. Under the condition of OGD but not in non-OGD condition, alteration of miR-181b expression level by transfection with pre-miR-181b or anti-miR-181b significantly affected N2A cell survival rate(-n=6). Accordingly, the changes of miR-181b levels significantly altered HSPA5 protein levels(n=3), but not the expression levels of HSPA5 mRNA. In addition, the results of luciferase reporter assay indicated that co-transfection of the luciferase reporters with pre-miR-181b or anti-miR-181b resulted in the inhibition or enhancement of the luciferase activities of luciferase expressing plasmid containing 3’-UTR of HSPA5 mRNA(n=5). Conclusions miR-181b plays an important role in N2A cell ischemic injury through negatively regulating HSPA5 protein level, which may provide a potential therapeutic target for ischemic stroke in miRNA levels.

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    Protective effects of ethyl acetate extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons induced by lipopolysaccharides
    HU Yu CHEN Xiao WANG Li-an GUAN Zhen-long WANG Yan-qin*
    2013, 44 (5 ):  621-625.  doi: 10.3969/j.issn.0529-1356.2013.05.007
    Abstract ( )  

    Objective To investigate the protective effects of different doses of Ethyl Acetate (EA) Extract from Chroogomphus rutilus on the degeneration and death of dopaminergic neurons of the PD mouse which was induced by lipopolysaccharides (LPS). Methods Adult male Kunming(KM) mice were used in the present study, LPS was injected into the substantia nigra compacta(SNc) to duplicate mouse model of PD. A tatal of 30 KM mice were randomly divided into five groups: the sham group, the LPS control group, low dose experimental group(LPS+100mg/kg), medial dose experimental group (LPS+200mg/kg) and high dose experimental group(LPS+400mg/kg), six mice per group. The immunofluorescence methods were used to label the dopaminergic neurons and astrocytes in SN. The dopaminergic positive neurons were counted and analyzed with statistics. Results The dopaminergic neuron numbers in LPS control group were markedly decreased in comparison with sham group (P<0.05), and the dopaminergic neurons appeared ovale, and the neurite was shorter than the sham group. Astrocytes displayed the activated state with larger bodies, thickened and shortened enation in the SN of LPS control group compared with the sham group. The dopaminergic neurons were increased significantly in the low and median dose groups compared with LPS control group(P<0.05), the density of astrocytes was lower. There was no significant difference of dopaminergic neurons between high dose group and LPS control group(P>0.05). Conclusion The low and median groups may protect the degeneration of dopaminergic neurons in SNc by depressing the activity of astrocytes in SNc.

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    Effects of transient focal cerebral ischemia on proliferation of neuronal precursors in adult rats subgranular zone
    WANG Lei JIN Guo-hua* ZHANG Xin-hua QIN Jian-bing
    2013, 44 (5 ):  626-629.  doi: 10.3969/j.issn.0529-1356.2013.05.008
    Abstract ( )  

    Objective To investigate proliferation changes of neuronal precursors in subgranular zone (SGZ) of adult rats after transient focal cerebral ischemia.
    Methods Ischemia was induced by occlusion of the middle cerebral artery in rats for 90min. The rats were given intraperitoneal injection of BrdU on the 1st and 2nd day after injury, and then were sacrificed on the 3rd, 7th, 14th and 28th day respectively, 6 rats in time point. The brains were removed and frozen sections were cut. Immunofluorescent staining was applied to observe the expression of Brdu, DCX, ki67, GFAP and CNP in SGZ. Results (1)The Brdu(+) cells assembled into clusters. The number of clusters increased at 3d and reached to peak at 7d,and then decreased gradually.(2)The cells in clusters increased at 3d and reached to peak at 7d, and then decreased gradually.(3)The rate of ki67(+) cells decreased from 3d to 28d. Conclusion MCAO/R induces proliferation of neuronal precursors in SGZ. Some of the progeny cells retain their ability to proliferate, and the others differentiate into neurons.

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    Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage
    ZHANG Qing JIANG Yan-xia LI Guo-liang WANG Qi-xin MA Cheng-tai* WANG Shou-biao YAO Ru-yong
    2013, 44 (5 ):  630-634.  doi: 10.3969/j.issn.0529-1356.2013.05.009
    Abstract ( )  

    Objective To investigate the effect of Xing-naojing and Shengmai injection on protease activated receptor-1(PAR1) expression in rat brain tissue after intracerebral hemorrhage (ICH).Methods  The ICH model was induced by injecting blood of its own. Forty male SD rats were randomly divided into ICH group (n=10), saline group (NS, n=10), Xing-naojing and Shengmai injection group (XNJSM, n=10), hirudin group (HIR, n=10). Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes. The expression of PAR1 in perihematomal tissue was examined by immunohistochemistry and Western blotting techniques. Results The expression of PAR1 in perihematomal tissue was increased after ICH. However,compared with ICH group, perihematomal tissue morphological changes were improved obviously. The expression of PAR1 was decreased significantly between XNJSM group and HIR group(P<0.05). Conclusion  XNJSM injection may play a neuroprotective effect by inhibiting the expression of PAR1 protein after ICH.

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    Effects of nitric oxide on netrin-1 expression and migration of astrocytes
    SHI Yue ZHANG Ye SHAO Jie YAO Yang-ming XIA Chun-lin*
    2013, 44 (5 ):  635-640.  doi: 10.3969/j.issn.0529-1356.2013.05.010
    Abstract ( )  

    Objective To investigate effects of NO on netrin-1 expression and migration of astrocytes. Methods Astrocytes were treated with a certain concentration of NO donor sodium nitroprusside (SNP) in vitro.The astrocyte migration was observed and the expression of netrin-1 at the different times after treatment was detected. Purified cultures of astrocytes were prepared from neonatal rats, based on the differential properties of developmental time-course and cellular adhesions. Astrocytes were inoculated to culture plates and were divided into experimental and control groups (n=6).The experimental groups were treated with 50μmol/L NO donor SNP. Cell migration was observed by scratching. The expression of netrin-1 after treatment was examined by Western blotting and immunocytochemistry. Results The speed of astrocyte migration increased after SNP treatment. The expression of netrin-1 in the protein level began to increase at 6 hours and reached the peak at 48 hours, compared with the control group (P<0.01), and then declined. Conclusion SNP may increase the expression of netrin-1 protein and promote astrocytes migration, indicating that netrin-1 promoting astrocytes migration maybe controled by NO.

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    Localization and clinical significance of kindlin-1 in human gingival epithelia
    XIE Ting-ting MEI Fang ZHONG Jin-sheng TANG Yan ZHANG Hong-quan*
    2013, 44 (5 ):  641-645.  doi: 10.3969/j.issn.0529-1356.2013.05.011
    Abstract ( )  

    Objectiv To investigate distributions of kindlin-1 in human normal gingival epithelia and chronic inflammatory gingival epithelia and establish clinical relevance between kindlin-1 and periodontal diseases.Methods Immunohistochemistry and Western blotting techniques were applied to detect the localization and expression of kindlin-1 of 10 human normal gingival epithelia and 8 chronic inflammatory gingival epithelia. Results Kindlin-1 was mainly localized in basal layer cells and upper granular layer cells of normal gingival epithelia, especially highly expressed in cytoplasm in granular layer cells, with some diffuse staining in the nuclei of suprabasal layer cells. In addition, the higher immunoractivity for kindlin-1 was observed in interdental gingiva than in free marginal gingiva (P<0.05). Compared to normal gingival epithelia, kindlin-1 presented much wider and higher expression in chronic inflammatory gingival epithelia (P<0.05). Western blotting further verified that kindlin-1 in chronic inflammatory gingival epithelia was higher expressed than in normal gingival epithelia (P<0.05). Conclusion The localization and expression of kindlin-1 in human gingival epithelia are closely associated with the differentiation and inflammation.

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    Biological Characteristics of adipose-derived mesenchymal stem cells after cryopreservation in vitro
    LI Qiong YANG Ci-qing GUO Zhi-kun* ZUO Hong-Bo LI Ci-xia LI He
    2013, 44 (5 ):  646-650.  doi: 10.3969/j.issn.0529-1356.2013.05.012
    Abstract ( )  

    Objective To explore the effect of cryopreservation on the biological and differentiation potential of mouse adipose-derived mesenchymal stem cells (ADMSCs)in vitro. Methods ADMSCs were isolated from mice fatty tissue and then cultured. The ADMSCs of third passage were cryopreserved in liquid nitrogen (-196℃) for 12 months as the experimental group. After recovery, a MTT method was applied to get ADMSCs growth curve. SA-β-Gal staining was used to detect ADMSCs senescence. Immunocytochemical method was used to detect the expression of cell surface molecules, such as CD73, CD90 and CD105. After induction, immunofluorescence was to applied to detect the expression of cardiac-specific troponin (cTnT). Alizarin red, alkaline phosphatase and oil red O stainings were used to detect the differentiation potential to osteogenic and adipogenic, respectively. Real time-PCR was used to detect the mRNA expression of cTnT, Gata4, Ost and Runx2. ADMSCs without cryopreservation were used as the control group. Results ADMSCs growth and senescence index were no significant difference between cryopreservation group and non-cryopreservation group (P>0.05). After induction, there was no significant difference in the expression of cTnT and the positive reaction of alizarin red, alkaline phosphatase and oil red O staining between two groups(P>0.05). Conclusion Cryopreservation has no significant effects on the biological characteristics of ADMSCs in vitro, including the basic biological properties and differentiation potential into cardiomyocytes, adipocytes and osteoblasts.

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    Relationship between UNC5H3,DCC, proliferation and prognosis in gastric cancer
    CHEN Jin-ming WANG Shen-hua WU Ding-qian DAI Meng-zhao HAN Bao-san*
    2013, 44 (5 ):  651-655.  doi: 10.3969/j.issn.0529-1356.2013.05.013
    Abstract ( )  

    Objective To investigate dependence receptors UNC5H3 and DCC in gastric cancer tissues and their association with proliferation, biological features, survival, and prognosis. Methods Tissue micro-array and immunohistochemistry were used to detect expression of UNC5H3, DCC and Ki-67. Integrated absorbance of stained sections was measured by Image-Pro Plus 6.0,and the expressional analysis was also verified.The relationship between UNC5H3, DCC and Ki-67 as analyzed.Survival was evaluated by Kaplan-Meier survival curves. Cox regression analysis was performed to evaluate the possibility of UNC5H3 and DCC as independent prognostic factors for gastric cancer. Results The positive-expression rate of UNC5H3, DCC and Ki-67 in tumor tissues from 60 patients with gastric cancer were 43.3%, 53.3% and 60.0%, respectively. UNC5H3 and DCC expressions were related to lymph node metastasis and distant metastasis (P<0.05). No correlation between UNC5H3, DCC and K-i67 was observed (P<0.05). Using Kaplan-Meier survival curves and the Log-ranktest, there was correlation between UNC5H3 and survival (P<0.05). No correlation between DCC and survival was observed (P>0.05). Using Cox regression model, UNC5H3 and DCC expressions were not significantly correlated with the prognosis of gastric cancer. Conclusion UNC5H3 and DCC may be related with tumorigenesis and tumor progression. The detection for UNC5H3 and DCC may be helpful to evaluate the clinicopathological parameters. The detection for UNC5H3 may also be helpful to evaluate survival time. However, UNC5H3 and DCC may not be an independent prognostic factor for gastric cancer.

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    Influence of annexin A7 low expression on proliferation of HepG2 cells
    WANG Xiao-jie LI Xin*
    2013, 44 (5 ):  656-660.  doi: 10.3969/j.issn.0529-1356.2013.05.014
    Abstract ( )  

    Objective To study whether annexin A7(ANXA7) low expression can affect proliferation of HepG2 cells. Methods Western blotting was used to identify siRNA can highly surpress annexin A7 expression. When the effect of the siRNA was assured, the siRNA was transfected into HepG2 cells by lipofectamine transfection method. At the same time, cells in negative control group were transfected negative siRNA while cells in blank control group only added lipofectamine 2000. Twenty-four, forty-eight and seventy-two hours after transfection ,cell counting was used to draw growth curve. Forty-eight hours after transfection, MTT assay was used to detect proliferation activity; Immunohistochemistry was applied to observe the expression of cyclinD1 and Ki67; Western blotting was performed to detect the expression of cyclinD1. Results Cell counting and MTT assay showed that proliferation of the siRNA interference group decreased significantly(P<0.05). The expression of cyclinD1 and Ki67 decreased compared with the negative control group and the blank control group(P<0.05). Conclusion ANXA7 low expression may inhibit proliferation of HepG2 cells.

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    Image anatomy of internal capsule in hunman brain
    QIN Yuan CHEN Nan GUO Yu-lin* LI Kun-cheng
    2013, 44 (5 ):  661-664.  doi: 10.3969/j.issn.0529-1356.2013.05.015
    Abstract ( )  

    Objective To study the anatomy characteristics of the internal capsule in the normal adults of Han nationality. Methods One thousand (2000 cerebral hemispheres) healthy Chinese Han adults were divided into 18-30,31-40,41-50,51-60,61-80 years old age groups, 100 males and 100 females per group.All the subjects underwent 1.5T MR scans using T1 weighted three-dimensional magnetization prepared rapid acquisition gradient echo sequence,and using 3D analysis software (Midobl) manual lays out the various anatomical value, the software automatically calculates its anatomical parameter values.
    Results 1. The anteroposterior diameters of left and right anterior limbs were (16.93±1.67) mm and (16.60±1.72) mm,left and right posterior limbs were (28.42±1.92) mm and (27.68±1.81) mm. 2. The lateral dimensions of left and right anterior limb were (11.25±1.52) mm and (11.10±1.53) mm and that of the left and right posterior limb were (20.10±2.33) mm and (19.44±2.17) mm. 3. The standard axial plane, the angle between the anterior limb and posterior limb of left and right internal capsule respectively were (125.66±2.38)°, (126.01±34.11)°. Conclusion High-resolution MRI can provide more precise images of the internal capsule and measurement data. This study provides reference ranges of the internal capsule in normal adults of Chinese Han.

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    Microscopic anatomical landmarks of the middle ear for the localization of the facial nerve
    CAI Zhi-yi LI Zhi-hai TAO Bao-hong JIN Qiao-zhi CHU Hong-juan YE Hua-fu*
    2013, 44 (5 ):  665-669.  doi: 10.3969/j.issn.0529-1356.2013.05.016
    Abstract ( )  

    Objective To observe the microdissection structure of the facial nerve in middle ear surgery and to clarify its clinical significance.Methods Microdissection was performed on 30 Chinese adult wet skulls via the transmastoid approach.The location of the facial nerve was identified by its stationary anatomic landmarks nearby. Resules The distances of both ends of the mastoid segment of the facial nerve to the imaginary line of the position were (-0.11±0.36)mm and (-0.14±0.38)mm, respectively. The lower edge of the tympanic segment of the facial nerve to the imaginary line of position was (0.31±0.12)mm. The trailing edge of short limb of incus to the lower edge of the lateral semicircular canal was (1.73±0.24)mm. The lateral semicircular canal and the trailing edge of short limb of incus to the upper edge of cavity segment of facial nerve were (2.65±0.25)mm and (2.20±0.41)mm, respectively. The upper edge of stirrup bone to the horizontal segment of facial nerve was (2.24±0.23 )mm. Conclusion Important anatomic landmarks for middle ear surgery include the lateral semicircular canal, short limb of incus, incustympanic ring, digastric ridge, vestibular, cochleariform process, oval window, pyramidal eminence, and chorda tympani nerve. These structures are closely related with the facial nerve. Thus, these anatomical features may paly an important role in localizing the facial nerve, avoiding iatrogenic injury, and improving operative quality.

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    Three-dimensional sectional imaging anatomy of the optic chiasma
    XU Xiang-dang REN Chuan-gen LI Jian-ce QIAO Hui-huang YANG Xin-dong CHEN Zhong-xiao CHEN Cheng-chun* FU Sheng-qi*
    2013, 44 (5 ):  670-674.  doi: 10.3969/j.issn.0529-1356.2013.05.017
    Abstract ( )  

    Objective To study the position,shape,adjacent structures and change rule of the adult optic chiasma, and to obtain its three-dimensional data of distance and angle from the serial thin transverse, sagittal, and coronary planes. Methods The three-dimensional data of distance and angles were collected from on 20 sets of serial transverse, sagittal, coronary sections of Chinese adult cadaveric heads and from 40 sets of serial transverse, sagittal, and coronary MRI of normal adult living subjeats. Transverse,superoinferior and anteroposterior diameters of the optic chiasma, anterior, lateral and posterior angles of the optic chiasma were measured. Results On the transverse plane, optic intracranial segment, optic chiasma and initial part of the optic tract were clearly displayed. The chiasma was transversed between the optic and infundibular recesses of the floor of the third ventricle and it located inferio to the A1 segment of the anterior cerebral artery, superior to the tuber cinereum and the pituitary stalk, and lateral to the C2 or C3 segment of the internal carotid artery. The mean transverse diameter in transverse section(TDT) of the optic chiasma was (13.54±3.12)mm , anteroposterior diameter in transverse section(APDT) was (7.91±1.29)mm , superoinferior diameter in coronal section (SIDC) was (3.51±0.49)mm, anterior angle(AA) was (69.7±9.1)°, left lateral angle(LLA) was (121.1±16.9)°, right lateral angle (RLA)was (122.3±15.2)°, and posterior angle(PA) was (73.3±10.2)°.There was no significant difference between the sectional data and MRI data by statistics. Conclusion Examination of the serial thin transverse, sagittal, and coronary planes and MRI is an effective methods to study and identify the spatial relation and the change rules of the optic chiasma.

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    Effects of mitochondrial metabolic activity and mitochondrial DNA replication on maturation, fertilization and developmental competence in vitro of rat oocytes
    GE Hong-shan* LI Xiao-he ZHANG Fan CHEN Hua XI Hai-tao HUANG Jian-ying ZHU Chun-fang Lü Jie-qiang
    2013, 44 (5 ):  675-679.  doi: 10.3969/j.issn.0529-1356.2013.05.018
    Abstract ( )  

    Objective To investigate the roles of mitochondrion on whether either mitochondrial DNA copy number or its metabolic capacity is an essential factor for both oocyte maturation and embryo development. Methods Both the metabolic inhibitor (FCCP) and the inhibitor of mitochondrial DNA replication(ddC) were introduced into the culture medium in order to analyze the affection of mitochondria rates of germinal vesicle breakdown. Nuclear maturation, fertilization and blastulation were assessedin vitro. Results The inhibiting mitochondrial DNA replication did not coincide with the mitochondrial metabolic capacity in oocytes. FCCP treatment significantly reduced the rate of nuclear maturation of oocytes and the development potential of embryos compared to untreated controls. Treatment of ddC did not affect maturationin vitro of oocytes, but impaired blastulation. Neither FCCP nor ddC treatment had an effect on the rate of fertilization. Conclusion Inhibiting mitochondrial metabolic activity during oocyte maturation has a negative impact on oocyte maturation and ensuing embryo developmental competence. Reduction in mitochondrial DNA copy number mainly affects embryonic development potential, but has little effect on oocyte maturation and fertilizationin vitro.

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    Effects of maturation in vitro on mitochondrial DNA copy and functions in oocytes
    GE Hong-shan* LI Xiao-he ZHANG Fan CHEN Hua XI Hai-tao HUANG Jian-ying ZHU Chun-fang Lü Jie-qiang
    2013, 44 (5 ):  680-684.  doi: 10.3969/j.issn.0529-1356.2013.05.019
    Abstract ( )  

    Objectiv To determine whether in vitromaturation (IVM) has an impact on oocyte’s mitochondrial number, and functions, and on oocyte’s quality.
    Methods The maturation mice oocytes were collected following either in vitro or in vivo (IVO) maturation process. The copies of mtDNA, the magnitude of mitochondrial membrane potential (ΔΨm) and oocyte adenosine triphosphate (ATP) content, pattern of mitochondrial distribution, reactive oxygen species(ROS)level, and the integrity of spindle and chromosome alignment were evaluated by RT-PCR, immunofluorescence and fluorescence-luciferase mensuration. Results Significant differences were detected in the copies of mtDNA,ROS level and integrity of cytoskeleton in oocytes between IVM and IVO.There were no significant differences in mitochondrial distribution, oxidative phosphorylation function in oocyte between IVM and IVO. Conclusion The in vitro non-physiological maturation may inhibit mtDNA replication, and increase ROS production and rates of abnormal spindle and chromosome alignment. This may partly explain the low development potential of IVM oocyte.

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    Protective effect of astragalus polysaccharides in model rats of doxorubicin induced cardiac injury
    GUAN Peng XU Bing-yuan LI Ya-qing GUO Qiu-hong WANG Na*
    2013, 44 (5 ):  685-688.  doi: 10.3969/j.issn.0529-1356.2013.05.020
    Abstract ( )  

    Objective To determine the protective effect of astragalus polysaccharides (APS) in doxorubicin induced heart faiure(HF). Methods Thirty male Sprague-Dawly rats were randomly divided into three groups: control group, HF group and APS group. APS group received APS [3g/(kg〖DK〗·d]for 14 days. HF group and APS group received doxorubicin 10.4mg/kg every 2 days for 4 times. Ventricular remodeling,apoptosis, and antioxidant indices were examined after the last intragastric administration. Results APS protected heart from myocardial fibrosis, myofibrils dissolve and broke, and mitochondria swelling. Evident cardiac damage induced by doxorubicin was revealed by hemodynamic changes and TUNEL staining compared to the control group. APS treatment caused significant attenuation of malonyldialdehyde(MDA)and Bax express. Superoxide dismutase (SOD) activity and Bcl-2 expression were significantly increased by APS in heart failure rats. Conclusion APS decreases doxorubicin-induced cardiotoxicity.

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    Increased expression of lxrα protein in renal tubular epithelial cells of crescentic glomerulonephritis
    WANG Jian-ping ZHANG Lian-shan* FENG Lu-lu ZHANG Yi-ming SHI Yong-hong NATORI Yasuhiro DUAN Hui-jun
    2013, 44 (5 ):  689-693.  doi: 10.3969/j.issn.0529-1356.2013.05.021
    Abstract ( )  

    Objective To observe the change of expression of liver X receptorα(LXRα) protein and mRNA in crescentic glomelonephritis(GN). Methods Fourty male Wistar Kyoto rats were randomly divided into control group and GN group. Crescentic GN was induced by once intravenous injecting of rabbit anti-rat glomerular basement membrane(GBM) antibody in 20 rats as GN group. Proteinuria/24 hours were measured on days 3,7, 14, 28 and 49. Expression of LXRα protein in the kidney on days 3, 14, 28 and 49 was detected by immunohistochemistry, and LXRα protein and mRNA in renal cortex on day 14 were analysed by Western blotting and reverse transcription real time polymerse chain reaction (Real time-PCR). Results On days 14 after injection of GBM, crescent formation arrived 65% , and GN rats showed massive proteinuria(167.03±42.50)mg/24hours and marked lipid accumulation in renal tubular epithelial cells. There was extensive expression of LXRα protein in renal tubular epithelial cells of GN rats on day 14. No positive expression of LXRα protein was observed in macrophages and glomerular cells including mesangial cells, podocytes and endothelial cells. The expression of LXRα protein was up-regulated in renal cortex of GN rats on day 14 than that of normal rats. There was no significant difference of the LXRα mRNA level between two groups. Conclusion We provided for the first time the evidence that expression of LXRα protein in renal tubular epithelial cells of crescentic GN was increased. This finding suggests that LXR signaling pathway may be involved in the pathophysiologic process of this GN.

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    Expression of neuronal nuclear antigen and neuron-specific enolase in the developing spinal cord of human embryos
    LIU Xue-hong ZHANG Yong CHEN Jian-er*
    2013, 44 (5 ):  694-698.  doi: 10.3969/j.issn.0529-1356.2013.05.022
    Abstract ( )  

    Objective To investigate the neuronal nuclear antigen(NeuN) and neuron-specific enolase (NSE) distribution in the developing stage of the human embryonic spinal cord and their clinical significance. Methods Sixteen cases of human embryos, from 2 to 4 month-age were used in the present study. Immunohistochemical staining was uesd to detect the expression and distribution of NeuN and NSE in the anterior and posterior horn of the spinal cord.
    Results At the second month of gestation, NeuN negative expression in posterior horn and NSE positive expression in some cells of the posterior horn were observed. At the third months, NeuN positive expression was first in some cells, and NSE was widely expressed in the posterior horn. At the fourth month, the number and intensity of NeuN positive expression were higher than before, and NSE positive expression was similar to that at the third month. From the second to fourth month of gestation, NeuN and NSE positive expressions were extensive in the anterior horn of human embryonic spinal cord.
    Conclusion NeuN and NSE positive expressions are appeared earlier in the anterior horn than posterior horn. From the second to fourth month of gestation, NeuN and NSE may regulate neurons in the mature process during the growth and development of human embryonic spinal cord.

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    Age variation of morphological traits in the head and face of Gejia people
    ZHENG Lian-bin* LI Yong-lan FENG Chen-lu RONG Wen-guo QI Xiao-lin NI Xiao-lu
    2013, 44 (5 ):  699-706.  doi: 10.3969/j.issn.0529-1356.2013.05.023
    Abstract ( )  

    Objective To study the change law of head-face morphological characters with the increasing age. Method Thirty-eight physical indices of head and face of 311 Gejia adults (159 males, 152 females) were investigated in Huangping county of Guizhou province. Twelve physical indices of head and face were calculated. Preliminary analysis was carried out on the age change of head-face morphological characteristics. Results With increasing age, the Mongoloid fold rate decreased, eye color became shallow, the height of the narrow part of the upper lip raised, and lip thickness decreased. Correlation analysis showed that morphological facial height, upper lip height, physiognomic ear length, and physiognomic ear breadth were positively correlated with age. In contrast, head breadth, external biocular breadth, lip height, and thickness of the lips were negatively correlated. The minimum frontal breadth, bigonial diameter, interocular breadth, mouth breadth, nasal depth showed no correlation with age variation. With increasing age, physiognomic facial height, morphological facial height, transverse cephalo-facial index, and vertical cephalo-facial index were ascending. Moreover, cephalic index, length-height index of head and lip index were declining. Conclusion With the growth,the head and face measurements, index of craniofacial characteristic and the proportion of observed indices change with regularity.

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    Physical Characteristics of Zhejiang Han
    LI Yong-lan LU Shun-hua ZHENG Lian-bin* ZHANG Yu-ke LI Chuan-gang ZHAO Man LUAN Tian-shu
    2013, 44 (5 ):  707-716.  doi: 10.3969/j.issn.0529-1356.2013.05.024
    Abstract ( )  

    Objective To study the physical characteristics of Zhejiang Han, and analyze the systematic status of Zhejiang Han in Mongoloid types. Method Using the method specified in the international academic community, the study investigated 86 physique targets for adults including 330 males in Zhejiang province (144 urban and 186 rural males) and 357 females (153 urban and 204 rural females), calculated 24 physique indices, counted index types, compared data with Chinese ethnic groups, and preliminarily analyzed the physique characteristics of Zhejiang Han. Results The Zhejiang Han (male and female)had a low percentage of eyefold of the upper eyelid, and a low percentage of Mongoloid fold. Most of the opening height of eyeslits were narrow, external angle was higher than internal angle, most of the nasal root height were middle size, straight nasal profile, most of zygomatic projection were tiny in male while middle in female, the nasal base was level, mostly oblique maximal diameter of nostrils, height of alae nasi was middle and wide breadth of alae nasi; round Lobe types, low size upper lip skin height, higher rate of middle size thickness of lips in male and highest rate of middle size thickness of lips and thin the second in female, black hair color, yellow skin color, brown eye color. Overall, the anthropometry indicators of head and face of Zhejiang Han were close to North Asia ethnic groups. The indices of head and face ranged between North Asia ethnic groups and South Asia ethnic groups. The anthropometry indicators of body were close to North Asia ethnic groups. Indices of body were not only close to North Asia ethnic group, but also to South Asia ethnic groups. Judged from the indices’ mean, both male and female of Zhejiang Han were brachycephaly, hypsicephalic type, metriocephalic type, euryprosopy and mesorrhiny. Judged from the indices of body, both male and female of Zhejiang Han were long trunk, narrow shoulder breadth, medium distance between iliac crests. Male was medium chest circumference, female was narrow chest circumference. Urban and rural male and urban female were mesatiskelic type, while rural female was subbrachyskelic type. Conclusion The physique of Zhejiang Han is close to North Asia ethnic group. The results of cluster analysis show that the 9 Han ethnic groups fail to cluster together. The clan source of Han ethnic groups is diversity, which leads to large differences in physique among different areas. It may not be appropriate to classify the whole Han ethnic groups loosely as a physical type.

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    Radiologic measurement and sexual diagnosis of finger length in Kazak nationality by discriminant analysis
    NNUERMAIMAITI·Bahaxiaer* AI Hua WU Hai-zhong
    2013, 44 (5 ):  717-721.  doi: 10.3969/j.issn.0529-1356.2013.02.025
    Abstract ( )  

    Objective To study the dependence between finger length and height of the Kazak adult in Xinjiang. Methods Healthy Kazak adults (male 100, female 100), with both hands for the X\|ray films were randomly selected and the corresponding finger length and height were measured. The length of the mean, standard deviation and standard error, of the phalanx length were calculated and inferred from the phalanx length height gender discrimination equation.
    Results There were significantly differences between male and female phalanx length (P<0.01). Based on the correlation analysis to establish the Kazak adult fingerlength inferred that the three gender discrimination equation of height(Y1=0.780ZM1+3.146YM2+1.594YS1+0.618ZS3+0.045YX1+1.891ZX1+2.548ZX3-135.324, Y2=0.3450ZM1+2.328YM2+1.814YS1+1.355ZS3+0.714YX1+1.461ZX1+1.580ZX3-118.678, Y3=3.540SG+0.859YM2-1.882ZS2+0.640YZ1-5.598YZ2+5.794ZZ2+0.807YZ3+0.695ZX1-391.229, Y4=3.284SG+0.313YM2-1.595ZS2+1.113YZ1-3.869YZ2+4.959ZZ2+5.244YZ3+0.353ZX1-340.001). Conclusion The discriminating formulas of this paper may be of great value in forensic medicine and anthropology of Kazak in Xinjiang.

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    Transforming growth factor-β superfamily signaling and its role in mouse early development
    GUO Hao XU Jian*
    2013, 44 (5 ):  722-728.  doi: 10.3969/j.issn.0529-1356.2013.05.026
    Abstract ( )  

    Transforming growth factor-beta (TGF-β) superfamily signaling has been a hot spot in recent years. TGF-β superfamily ligands bind to their receptors which activate SMADs. The SMAD proteins form a complex in the cytoplasm, which enters the nucleus through the nuclear pore complex, regulating the expression of specific genes. TGF-β superfamity signaling has a lot of functions relating to fibrosis, tumorigenesis and immunoregulation. TGF-β superfamily signaling plays a key role in the early development of mice. In this review, the characteristics of TGF-β superfamily signaling pathway are discussed. We focus on how this signaling influences mouse early embryonic development.

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