Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages

doi:10.16098/j.issn.0529-1356.2015.02.015

Abstract

Abstract:

Objective To investigate the distribution of gene product 9.5 and neuropeptide Y(NP-Y) in yak testis and in an attempt of exploring their relationships with testicular spermatogenesis at different ages. Methods Using immunohistochemical SP technique, we depicted the distribution characteristics of PGP 9.5 and NP-Y in yak testis: 9 pairs of yak calves in age from 3 to 4 months, 10 pairs of young adult yak from 3 to 4 years and 7 pairs of aging yak from 9 to 13 years old were prepared by orchiectomy surgery, bedded in paraffin and sectioned serially. Results NP-Y and PGP 9.5 immunoreactivities were abundantly distributed in gonads, mainly associated with Leydig cell nests, blood vessels, and seminiferous tubules. PGP 9.5 and NP-Y were mainly expressed in spermatogonia of yak calves testis and in spermatogenic cells of young yak testis. By contrast, no NP-Y and PGP 9.5 immunoreactivities were observed within the primary spermatocytes. The expression of PGP 9.5 and NP-Y in corpus testis were stronger than the caput and cauda of the testis. A small number of PGP 9.5-immunoreactive cells was detected in testis vscellum of aging yak. Conclusion Our results suggest that the PGP 9.5 and NP-Y expression is significantly different in young adult yak testis and may serve as regulators for testicular functions.The significant dcrease of distribution of NP-Y in the wall of vascular structure in aging yak testis.

Key words: Protein gene product 9.5, , Neuropeptide Y, , Testis, , Age, , Immunohistochemistry, Yak

YUAN Li-gang* QU Ya-ling GU Lai-feng TIAN Dan-zeng. Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages[J]. AAS, 2015, 46(2): 232-237.

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