Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (6): 722-729.doi: 10.16098/j.issn.052-1356.2023.06.015

• Histology,Embryology and Developmental Biology • Previous Articles     Next Articles

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats

LIU Li-ping1 ZHU Meng-ni1 XU Hui2*   

  1. 1. Department of Obstetrics of the Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan430015, China; 

     2.Department of Obstetrics of Wuhan Children’s HospitalWuhan Maternal and Child Healthcare Hospital Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430015, China

  • Received:2022-07-21 Revised:2023-02-09 Online:2023-12-06 Published:2023-12-06
  • Contact: Hui XU E-mail:xuhui@zgwhfe.com

Abstract:

Objective To explore the effect of interleukin (IL)-6 on nucleated erythrocytes in lipopolysaccharide (LPS)-induced preeclampsia rats.  Methods ELISA and immunohistochemistry were used to detect the IL-6 in peripheral blood and placenta of preeclampsia and normal pregnancy; Flow cytometry and immunofluorescence were used to detect the maternal nucleated erythrocytes. Pregnant SD rats were randomly divided into 3 groups: the control, LPS and LPS +anti-IL-6 group; IL-6, the proportion of nucleated erythrocytes, JAK2/MEK and PI3K/Akt signal-related genes were detected.  Results The IL-6 of preeclampsia was higher than that of normal patients. Compared with the Control group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65, IL-1B mRNA of LPS group increased, the fetal weight decreased; Compared with the LPS group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65 and IL-1B mRNA of the LPS+anti-IL-6 group decreased.   Conclusion The up-regulation of IL-6 of preeclampsia patients is accompanied by increased nucleated erythrocytes in peripheral blood. Neutralizing IL-6 in vivo may down-regulate JAK2/PI3K/Akt/NF-κB-signal-mediated IL-1B to protect preeclampsia rats.

Key words: Preeclampsia, Interleukin 6, Nucleated erythrocyte, Interleukin 1B, Immunohistochemistry, Immunofluorescence, Human, Rat

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