Effect of hyperthermia to apoptosis on mice thymocytes

doi:10.3969/j.issn.0529-1356.2014.01.010

Abstract

Abstract:

Objective To study the effect of hyperthermia to apoptosis on mice thymocytes. Methods One-month-old male mice were divided into 6 groups randomly (A-F).5 mice per group, and 1 control group (A) and 5 experimental groups (B-F). Mice in experimental groups were stressed for 2 hours at 37℃ and then raised at room temperature(RT). At the different time points (0hour,2hours,6hours,10hours,20hours) after RT recovering, thymuses from one group were collected. Transmission electron microscopy was used to observe cellular morphology. Flow cytometry and hematoxylineosin (HE) staining were used to analyze apoptosis and cell cycle of thymocytes. Expression of proliferating cell nuclear antigen(PCNA) and heat shock protein(HSP70) were detected by immunohistochemistry. Results Typical morphology of apoptotic cells were observed under transmission electron microscopy at 2hours, 6hours and 10hours after hyperthermia. At 6hours after hyperthermia, thymocytes of S phase and apoptosis reached the max while thymocytes of G0/G1 phase and G2/M phase reduced to the minimum. At the same time point, PCNA expressed most. But HSP70 began to express at 0hours and expressed most at 2-6hours after hyperthermia. Conclusion Apoptosis of thymocytes was obviously effected by hyperthermia. HSP70 had protective effect during apoptosis.

Key words: Hyperthermia, Proliferating cell nuclear antigen, Heat shock protein, Thymocytes, Flow cytometry, Immunohistochemistry, Mouse

ZHANG Jin-hong ZHANG Lan WANG Fang* . Effect of hyperthermia to apoptosis on mice thymocytes[J]. AAS, 2014, 45(1): 53-57.

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