Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP

doi:10.3969/j.issn.0529-1356.2014.03.007

Abstract

Abstract:

Objective To explore the localization and expression of dopaminergic neurons in olfactory bulb of cynomolgus monkeys damaged by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）. Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of dopamine neurons (MPTP group) and three adult cynomolgus monkeys were as a control group. Immunohistochemical staining was performed to examine the localization and expression of dopaminergic neurons in the olfactory bulb in normal and MPTP group monkeys. The numbers of DA-positive and DARPP32-positive cells were counted and the average absorbance was measured in normal and MPTP group. Results DA and DARPP32 positive neurons were concentrated in the glomerular layer (GL) of olfactory bulb. DA positive nerve fibers were distributed in the GL while DARPP32 positive nerve fibers appeared in all layers, and most nerve fibers were in GL and external plexiform layers (EPL). After MPTP injury, compared with the normal control group, DA and DARPP32 positive neurons and nerve fibers decreased in MPTP group and DA neurons and nerve fibers decreased significantly. Conclusions DA neurons and nerve fibers are in the GL of cynomolgus monkey olfactory bulb. DA neurons and fibers are significantly reduced in the olfactory bulb of cynomolgus monkeys damaged by MPTP, which may be associated with the dysosmia in Parkinson’s disease.

Key words: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Olfactory bulb, Dopamine, Dopamine and adenosine 3‘5‘-monophosphate-regulated phosphoprotein, Immunohistochemistry, Cynomolgus monkey

CHENG Wei REN Zhen-hua GUAN Yun-qian WU Di YUE Feng LI Guang-wu*. Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP[J]. AAS, 2014, 45(3): 333-337.

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