Decrease of the glial and fibrotic scars tissue formation by degrading fibrinogen after traumatic brain injury

doi:10.3969/j.issn.0529-1356.2014.06.001

Abstract

Abstract:

Objective To investigate probability of reducing formation of glial and fibrotic scar by degrading fibrinogen after traumatic brain injury. Methods The nigrostiatal dopaminergic pathway was unilaterally transected in 8-weeks-old Kunming mouse according to the method of Kawano et al. Adult male mice were anesthetized and transferred to a stereotaxic frame. A small oblong hole at the right rear of the bregma at 1.5mm and at a depth of 6.0 mm from the surface of the brain was made with a dental drill.. The blade was slowly pulled out, bleeding was stopped and the incision was sutured. Twenty four Kunming mice were randomly divided into control group and experimental group. For three consecutive days, experimental group mice were injected with batroxobin at 1 hour after operation. The mice brains were obtained at 4days, 7days and 14days after brain injury. Immunohistochemical localization of fibrotic scar and glial scar were examined by using the antibodies collagenⅣ(ColⅣ) and GFAP. The formation of scar tissue was observed by double immunofluorescent staining. Results After 4 days injury, the lesion site of the control group appeared ColⅣ deposition,which was surrounded by glial limitans formed by reactive astrocytes. Compared with the fibrotic scar of the lesion center 7days after injury, more obvious ColⅣ deposition was found at 14days than 7days after injury, and the lesion center was surrounded by the reactive astrocytes. However, the glial limitans were not found around the lesion center in the experimental group and fibrotic scar was almost disappeared, but the reactive astrocytes surrounded the lesion center. Double immunofluorescent staining demonstrated that there was FN immunoreactivity deposition in the lesion site of control groups forming fibrotic scar, while the experimental group reduced the deposition of FN significantly 7days after injury, and almost eliminated it at 14days after injury. Both groups had the GFAP positive cells surrounded the lesion site. Conclusion After traumatic brain injury, injection of batroxobin could decrease the formations of the glial scar and fibrotic scar by degrading fibrinogen.

Key words: Traumatic brain injury, Fibrotic scar, Glial scar, Batroxobin, Immunofluorescence, Mouse

BAI Dan LI Dan LIU Nan PEI Dan LI Hong-peng*. Decrease of the glial and fibrotic scars tissue formation by degrading fibrinogen after traumatic brain injury[J]. AAS, 2014, 45(6): 729-734.

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