AAS ›› 2014, Vol. 45 ›› Issue (5): 656-662.doi: 10.3969/j.issn.0529-1356.2014.05.013

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Effect of lipopolysaccharide on the iron metabolism in macrophages

WANG Li 1, 2 JIANG Bao-hua4 QIAN Zhong-ming 1, 3 DUAN Xiang-lin 1*   

  1. 1. Institute of Neurobiology and Neuropharmacology, Hebei Normal University, Shijiazhuang 050016, China; 2. College of Bioscience and Bioengineering, Hebei University of Sciences and Technology, Shijiazhuang 050018, China;3. Laboratory of Iron Metabolism, Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Kowloon, Hong Kong, China; 4. Department of Basic Education, Hebei Political Science and Law Vocational College, Shijiazhuang 050061, China
  • Received:2012-06-12 Revised:2014-05-29 Online:2014-10-06 Published:2013-10-06
  • Contact: DUAN Xiang-lin E-mail:smartliwang@163.com

Abstract:

Objective To study the effect of lipopolysaccharide(LPS) on the activity of primary cultured macrophages and the distribution of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1). Methods Primary cell culture, MTT chromotest, cytochemistry chromotest and cell immunofluorescence techniques were used in this work.
Results DMT1 was mainly distributed in the cytoplasm, which illuminates that DMT1 mediates the macrophage intracellular transit of iron from phagolysosome to cytoplasm. FPN1 was distributed in the cytoplasm and membrane, and the cytoplasm was the main site of FPN1 distribution in macrophages. Conclusion Iron liberation from heme inside the phagolysosome occurs after erythrophagocytosis and it is possible that FPN1 mediates intracellular transit of iron released by heme catabolism. The study found that LPS promoted the cell growth and this effect was reached to the peak in the 10-5 μg/L LPS group, but the cell growth was blocked with the increase of LPS concentration.

Key words: Divalent metal transporter 1, Ferroportin 1, Primary culture, Macrophage, Mouse