Acta Anatomica Sinica

AAS ›› 2014, Vol. 45 ›› Issue (5): 663-669.doi: 10.3969/j.issn.0529-1356.2014.05.014

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Induction effect of hepatocyte growth factor and insulin-like growth factor on cardiac stem cells

ZHU Bei-bei 1,2 CAI Xin-hua1 SUN Yin-ping 2*   

  1. 1. Key Laboratory for Medical Tissue Regeneration of Henan Province, Xinxiang Medical University, He’nan Xinxiang 453003, China; 2. Department of Pathophysiology, Xinxiang Medical University, He’nan Xinxiang 453003, China
  • Received:2014-02-10 Revised:2014-03-24 Online:2014-10-06 Published:2013-10-06
  • Contact: SUN Yin-ping E-mail:syp2004@xxmu.edu.cn

Abstract:

Objective To investigate whether hepatocyte growth factor (HGF) and insulin-like growth factor (IGF1) induce cardiac stem cells (CSCs) to proliferate and directly differentiate into cardiomyocytesin vitro. Methods The myocardial tissues were dissected for primary culture of CSCs with the method of explants. The expressions of c-kit and CD34 were examined with immunofluorescence. Primary cells were purified with c-kit by flow cytometry. CFDA SE fluorescent probe was used to detect the proliferation of c-kit+CSCs. C-kit+CSCs were divided into two groups, and cardiac stem cells group and co-cultured with cardiomyocytes group, both group were cultured with HGF and IGF1. An inverted microscope was used to observe changes in cell number and morphology in different periods. Living cells workstation was used to observe CFDA SE fluorescence intensity, to acquire images and do statistical analysis. Immunofluorescence technique was used to detect the expression of Nkx2.5 and cardiac troponin T. Results In cardiac stem cells group，CSCs had no obvious changes in cell number. In co-cultured with cardiomyocytes group, CSCs proliferated and had changes in morphology. Nkx2.5 and cTnT were positively expressed. Several CSCs differentiated into beating cardiomyocytes. Conclusion In co-cultured with cardiomyocytes condition, HGF and IGF1 may promote CSCs to proliferate and differentiate into beating cardiomyocytes.

Key words: Cardiac stem cell, Proliferation, Cardiac differentiation, Hepatocyte growth factor, Insulin-like growth factor, Immunofluorescence, Rat