Effects of recombinant human endostatin on the expression of vascular endothelial growth factor, transforming growth factor-β1 and basic fibroblast growth factor on hypertrophic scar in rabbit ears

doi:10.16098/j.issn.0529-1356.2015.01.018

Abstract

Abstract:

Objective To investigate the effect of rhEndostatin on hypertrophic scar in rabbit ears and its mechanism. Methods Twenty healthy New Zealand big-eared white rabbits were divided equally into normal control group, model group, normal saline (NS) control group, rhEndostatin treatment group and triamcinolone acetonide (TA) control group. All groups were reproducing animal model of hyperplastic scar except normal control group, 1 cm ×1 cm size of the wound were made on ventral side of rabbit ears. Treatments were administered starting on postoperative days 28. rhEndostatin (100μl) was injected intradermally into the wounds of rhEndostatin treatment group with scar block local injection, NS control group injected with saline, the injection frequency of the two groups was once every other day for 6 successive times. The wounds treated with TA received the same doses, and the injection frequency was once a week, a total of 2 times. For model group, wounds received no injections. At postoperative days 47, collected scars and normal skin specimens, observe the change of rabbit ear scar in all groups, and the immunohistochemical method was also used to detect the distribution of vascular endothelial growth factor-β₁（VEGF）, transforming growth factor-β₁（TGF-β₁）and basic fibroblast growth factor（bFGF）positive signals. Results The scar morphological change appeared to be smaller, softer, flatter, and lighter in color in the rhEndostatin treatment group than those in the model group and NS control group. The intensities of immunohistochemical positive staining for VEGF and TGF-β1 were lower in rhEndostatin treatment group than those in the model group. The bFGF immunohistochemical positive staining intensity was increased significantly(P<0.01). Conclusion rhEndostatin can inhibit the hypertrophic scar in rabbit ears, the mechanism might be related to expression of VEGF, TGF-β₁ and bFGF.

Key words: Hypertrophic scar, Recombinant human endostatin, Vascular endothelial growth factor, Transforming growth factor β₁, , Basic fibroblast growth factor, , Immunohistochemical SP method, , Rabbit
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ZHANG Xiao-ming YU Jian HUANG Xue-ying* DENG Xue-fei LI Xiao-jing. Effects of recombinant human endostatin on the expression of vascular endothelial growth factor, transforming growth factor-β₁and basic fibroblast growth factor on hypertrophic scar in rabbit ears[J]. AAS, 2015, 46(1): 101-105.

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Effects of recombinant human endostatin on the expression of vascular endothelial growth factor, transforming growth factor-β₁and basic fibroblast growth factor on hypertrophic scar in rabbit ears

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