Structure and distribution of extracellular matrix proteins in yak calves testis

doi:10.16098/j.issn.0529-1356.2015.01.019

Abstract

Abstract:

Objective To characterize the structure and distribution of extracellular matrix proteins of testis in 9 yak calves. Methods Histochemistry and transmission electron methods were used to study the microstructure and ultrastructure of yak calves testis, and immunohistochemical SP technique and Image Pro Plu (IPP) statistics methods were used to identify the distribution of the laminin(LN), type Ⅳ collagen (ColⅣ) and heparan sulfate proteo glycans (HSPG).
Results The germinal epithelium of seminiferous tubule was constructed 1-2 layers by Sertoli cells and gonocytes without appearance of the lumen. The gonocytes were larger and abundant with cytoplasm.Sertoli cells were rich in heterochromatin and the microfilaments constructed by the typical ectoplasmic specialization and actin filament were accumulated in the submembranes regions between the adjacent Sertoli cells.Immunostaining analysis appeared that the extracellular matrix (ECM) proteins LN, ColⅣ and HSPG were weakly present throughout the basement membranes and peritubular myoid cells in yak calves seminiferous tubule.The relative expression of LN in the gonocytes was significantly lower than Col Ⅳ and HSPG（P<0.05）, and no expression in Sertoli cells and leydig cells. The strong immunoreactivity for Col Ⅳ and HSPG was seen in the Sertoli cells. In addition, the distribution of HSPG in the Leydig cells was higher than Col Ⅳ. Conclusion Taken together, the proliferation of the Sertoli cells is the main causes for the development of the yak calves seminiferous tubule and the Leydig cells are in the transition period which developed from embryonal type to a mature stage; the distribution of ECM proteins LN,Col Ⅳand HSPG may serve as a meaningful index for the further research of the yak testis development.

Key words: Testis, Collagen, Laminin, Heparan sulfate proteoglycans, Immunohistochemistry, Yak calve

YUAN Li-gang* QU Ya-ling ZHU Jun-feng GU Lai-feng TIAN Dan-zeng. Structure and distribution of extracellular matrix proteins in yak calves testis[J]. AAS, 2015, 46(1): 106-112.

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