Effects of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells

doi:10.3969/j.issn.0529-1356.2014.06.011

Abstract

Abstract:

Objective To investigate the effects and mechanisms of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells (BMSCs). Methods The porcine BMSCs were cultured and differentiated with cell culture and adipegenic differentiation medium containing different concentrations of curcumin, the effects of curcumin on proliferation of porcine BMSCs were detected by MTT, the effects of curcumin on adipogenic differentiation were analyzed by morphology and oil red O, and the expressions of peroxisome proliferator-activated receptor γ(PPARγ2) mRNA and lipoprotein lipase(LPL) mRNA were detected by Real-time PCR. Results When the porcine BMSCs were cultured with curcumin, 1μmol/L curcumin at the third days of culture and 5, 10μmol/L curcumin at the fifth days of culture significantly promoted cell proliferation (P<0.05), but curcumin concentration higher than 15μmol/L inhibited cell proliferation (P<0.05). When the porcine BMSCs were differentiated with curcumin, the different concentrations of curcumin significantly inhibited adipogenic differentiation (P<0.05). The inhibition rate of 0.5μmol/L curcumin was 28.3% and 20μmol/L curcumin was 71.24%. When the cells were treated with 20μmol/L curcumin and differentiated in 5, 10 and 15 days, the inhibition rates of expressions of PPARγ2 and LPL mRNA were 19.11%, 49.52%, 76.76% and 37.58%, 49.32%, 74.70% respectively.
Conclusion The proper concentrations of curcumin were able to promote the proliferation and inhibit adipogenic differentiation of porcine BMSCs, suggesting that curcumin may play an important role in the regulation of adipocyte differentiation.

Key words: Bone mesenchymal stem cell, Curcumin, Proliferation, Adipogenic differentiation, Real-time PCR, Porcine

ZHANG Qing-mei LI Fang-zheng JIANG Zhong-ling SUN Dong-xing LI Dong-jian SONG Xue-xiong*. Effects of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells[J]. AAS, 2014, 45(6): 793-799.

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