Effect of angelica polysaccharide on hematopiesis in aging rats and its biological mechanism

doi:10.3969/j.issn.0529-1356.2014.06.010

Abstract

Abstract:

Objective To explore the effect and mechanism of angelica sinensis polysaccharide（ASP）on hematopiesis in aging rats and to provide theoretical and experimental evidences for effective natural medicine of antiaging or protecting hematopoietic function. Methods Male SD rats（n=40）aging from 6 to 8 weeks old were randomly divided into normal group(NG, n=10)，ASP normal group(ANG, n=10), aging model group(AMG, n=10) and ASP aging model group(AAMG, n=10). After 2 days of the treatment, the eyeball blood was collected for the peripheral blood routine test; The femur was taken to count the number of each femur bone marrow mononuclear cells (BMNCs). The proliferation of BMNCs was detected by CCK-8. The distribution of cell cycles and reactive oxygen species (ROS) levels were analyzed by flow cytometry(FCM). The capability of colony formation was examined by CFU-Mix cultivation. The ratio of the SA-β-Gal staining positive BMNCs was counted. The total antioxidant capacity of the cell was detected by enzymatic assay. The aging related proteins of P53 and P21 were detected by Western blotting. Results Compared with the aging model group, in the ASP aging model group,the decrease in the amounts of the peripheral blood RBC, PLT and WBC was evidently inhibited; the proliferation of the BMNCs was enhanced;the colony formation of CFU-Mix was markedly increased; the percentage of SA-β-Gal, the ratio of G₁ stages and the product of ROS positive cells were significantly reduced; the ratio of S stages was markedly promoted; the total antioxidant capacity of the cell was significantly promoted; the expression of the aging related protein of P53 and P21 was evidently down-regulated.
Conclusion ASP can protect hematopoietic function from decline by antagonize D-galactose that induced oxidative stress, suggesting the mechanism of ASP protecting hematopiesis may regulate p53/p21 pathway.

Key words: Angelica polysaccharide, Aging model, Bone marrow mononuclear cell, Western blotting, Rat

ZHANG Yan-yan LI Jing JIA Dao-yong ZHANG Meng-si XU Chun-yan JING Peng-wei LI Cheng-peng WANG Lu WANG Shun-he WANG Ya-ping*. Effect of angelica polysaccharide on hematopiesis in aging rats and its biological mechanism[J]. AAS, 2014, 45(6): 785-792.

References

［1］Zhang XP, Wang QX, Wang YP, et al. Angelica sinensis polysaccharides delay aging of hematopoietic stem cells through inhibitting oxidative damage ［J］. China Journal of Chinese Materia, 2013, 38(3):407-412. (in Chinese)
    张先平,王乾兴，王亚平，等.当归多糖抑制氧化损伤延缓造血干细胞衰老［J］.中国中药杂志, 2013, 38(3):407-412.
［2］Yang TH, Mei QB, et al. Effects of Angelica polysaccharide on immunologic function of mice ［J］. Chinese Pharmacological Bulletin, 2003, 19( 4) :448-451. (in Chinese)
    杨铁虹, 梅其炳，等.当归多糖对小鼠免疫功能的影响［J］.中国药理学通报, 2003,19( 4) :448-451.
［3］Wang ShL, Wang YP, Dai Q, et al. Study on effect of angelica polysaccharideon modulation of human CFU-GM ［J］. Acta Anatomica Sinica, 2001,23（3）:241-245. (in Chinese)
    王莎莉,王亚平,戴勤,等.当归多糖对人粒单系造血祖细胞增殖分化的调控机理研究［J］.解剖学报,2001,23（3）:241-245.
［4］He XL, Guan XJ, Wu H, et al. Effects of angelica polysaccharide on the apoptosis and oxidative damage bone marrow mononuclear cells of ionizing radiation injured mice［J］.Journal of Chongqing Medical University, 2012,37(4):315-319. (in Chinese)
    何晓莉,关雪晶,吴宏,等.当归多糖对电离辐射致小鼠骨髓单个核细胞凋亡及氧化损伤的影响［J］.重庆医科大学学报,2012,37(4):315-319.
［5］Song ShD, Wang JW, Wang YP, et al. Experimental study on the effect of angelica polysaccharide on inhibition of proliferation and induced differentiation towards erythroid cell of human erythroleukemia cell line( K562) ［J］.Journal of Chongqing Medical University, 2008,33（1）:1-5. (in Chinese)
    宋姝丹,王建伟,王亚平,等.当归多糖对K562细胞增殖抑制及定向红系细胞分化的实验研究［J］.重庆医科大学学报,2008,33（1）:1-5.
［6］Zhang XP, Wang QX, Wang YP, et al.Angelica sinensis polysaccharides regulate aging of mice hematopoietic stem cell through cell cycle protein ［J］. Basic Medical Sciences and Clinics, 2013，33（3）：320-324. (in Chinese)
    张先平，王乾兴，王亚平，等.当归多糖对小鼠衰老造血干细胞细胞周期蛋白的调控［J］.基础医学与临床，2013，33（3）：320-324.
［7］Zhang XP, Liu J, Wang YP, et al. Effect of Angelica sinensis polysaccharide on expression of telomere，telomerase and P53 in mice aging hematopoietic stem cells［J］. China Journal of Chinese Materia, 2013, 38(14):2354-2358. (in Chinese)
    张先平，刘俊，王亚平，等. 当归多糖对小鼠衰老造血干细胞端粒、端粒酶及P53的影响［J］.中国中药杂志, 2013, 38(14):2354-2358.
［8］Song X, Bao M, Li D, et al. Advanced glycation in D-galactose induced 22 mouse aging model［J］. Mech Aging Dev, 1999,108(3): 239-251. 
［9］Wang YP, Wu H, Wang JW, et al. Hematopoietic Stem Cell Biology and Research Methods［M］.Beijing : Science Press, 2007：348-349. (in Chinese)
    王亚平,吴宏，王建伟，等.造血干细胞生物学及其研究方法［M］.北京：科学出版社，2007:348-349.
［10］Lee MY，Wang Y, Vanhoutte PM. Senescence of cultured porcine coronary arterial endothelial cells is associated with accelerated oxidative stress and activation of NF kappa ［J］. J Vasc Res，2009，47( 4) : 287-298.
［11］Michael WE, Wang H, Jeffrey AJ. Antioxidant-chemoprevention diet ameliorates late effects of total-body irradiation and supplements radioprotection by MnSOD-plasmid liposome administration ［J］. Radiat Res, 2011，175(6): 759-765.
［12］Sahin E, Depinho RA. Linking functional decline of telomeres, mitochondria and stem cells during ageing［J］. Nature, 2010, 464(7288):520-528.
［13］Dumble M, Moore L, Chambers SM, et al. The impact of altered p53 dosage on hematopoietic stem cell dynamics during aging ［J］. Blood, 2007, 109(4):1736-1742.
［14］Rai P，Onder TT，Young JJ,et al. Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence［J］. Proc Natl Acad Sci USA,2009,106(1) : 169-174.

[1]	YANG Meng-yi NIU Shi-bo ZHANG Jing DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index finger and ring finger length ratios and polymorphism of homeobox A11 gene locus among Ningxia college students [J]. Acta Anatomica Sinica, 2024, 55(1): 62-66.
[2]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[3]	XIE Hui-lan FANG Fang LIN Yi. Mechanism of Chir99021 regulating Wnt/β-catenin signaling pathway inhibiting osteogenic differentiation of rat dental pulp stem cells [J]. Acta Anatomica Sinica, 2024, 55(1): 67-72.
[4]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[5]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[6]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[7]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[8]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[9]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[10]	SU Peng WANG Xing-yi LIANG Jing-yan XIONG Tian-qing. Optimization and identification of a low density and high purity method for primary hippocampal neuron culture from fetal rats [J]. Acta Anatomica Sinica, 2024, 55(1): 113-119.
[11]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[12]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[13]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[14]	ZHAO Wei-ming LI Xiao YU Guo-ying WANG Gai-ping CHANG Cui-fang . Effect of serine protease inhibitor Kazaltype 1 on the proliferation of hepatocellular carcinoma cell and its mechanism [J]. Acta Anatomica Sinica, 2023, 54(6): 695-702.
[15]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.