Reelin and the cerebellar development—— the regulatory effect of Notch1 signaling pathways

doi:10.16098/j.issn.0529-1356.2015.02.007

Abstract

Abstract:

Objective To investigate the effect of Reelin on the neural migration, distribution of radial glial cells, cell migration and Purkinje cells’ development in the cerebellum, and to discuss. Regulatory effects of Notch signaling pathways. Methods A total of 148 wild-type (WT) and reeler mice from E9 (embryonic day 9) to P60 (postnatal day 60) were used for immunofluoresent labeling to visualize, the radial glial cells, neural stem cells, Purkinje cells and Notch1 positive cell in the developing cerebellum. Results Comparing with WT mice, in reeler mice, the orientations of radial glial cells (putative Bergmann cell) were disordered with number decrease (P<0.01). The cell bodies of Bergmann cells did not move into their proper position and lingered around in internal granular layer(IGL). Sox2-positive stem cells in external granular layer migrated into internal granular layer with time-lag. Purkinje cells did not reach their destination and accumulated in internal granular layer. We found that Notch1 positive cells reduced in reeler mice. Conclusion Reelin plays an important role in the development of radial glial cells (Bergmann cells), cell migration and neural proliferation. In addition, Notch1 signaling pathway is involved in cerebellar development, via the regulation to Reelin pathway.

Key words: Reelin, Notch1 receptor, Cerebellar development, Neural stem cells, Immunofluorescence, Mouse

YAN Ming-chao NIU Yan-li WANG Xiao-qing LIANG Shuang GUO Jun-nan SHI Shu-qin HU Sang DENG Jin-bo*. Reelin and the cerebellar development—— the regulatory effect of Notch1 signaling pathways[J]. AAS, 2015, 46(2): 182-189.

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