AAS ›› 2015, Vol. 46 ›› Issue (5): 690-693.doi: 10.16098/j.issn.0529-1356.2015.05.020

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Application of hydroxyethyl methacrylat plastic-embedded sectioning technology in the histological analysis of the brain tissue of mouse embryo

LI Shuai CHEN Yu-chen ZHANG Xue-xi TONG Xian-jie SONG Xiao-bing FAN Chun-e CHEN Hua-qun*   

  1. Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Jiangsu, Nan Jing 210023, China
  • Received:2015-02-03 Revised:2015-04-22 Online:2015-10-06 Published:2015-10-06
  • Contact: CHEN Hua-qun E-mail:chenhuaqun@njnu.edu.cn

Abstract:

Objective To study the application of plastic embedding technology in the histological analysis of the brain of mouse embryo. Methods The E13.5 embryos were fixed in 4% polyformaldehyde 4℃ overnight. The head was dissected and embedded in hydroxyethyl methacrylat (HEMA). The blocks were sectioned at 4μm thick and stained with HE staining. Results Compared with paraffin embedded sections, the HEMA embedded brains showed much better morphologic and structure preservation, as well as higher quality of HE staining images. Conclusion The HEMA plastic embedding is better than paraffin embedding in the histological analysis of the brain tissue of the mouse embryo.

Key words: Plastic embedding, HEMA, Mouse embryo, HE staining