Mathematical model construction and analysis of the effect of estradiol on the electrical activity of gastric antrum in female rabbits

doi:10.16098/j.issn.0529-1356.2020.03.007

Abstract

Abstract:

Objective To investigate the expression of estrogen receptor in gastric antrum and the effect of estrogen on electrical activity of gastric antrum smooth muscle in female rabbits in virtue of the constructed mathematical model in order to explore the regulation of estrogen on gastric motility. Methods Using immunofluorescence to observe the expression of estrogen receptor in gastric antrum. Using BL-420F bio monitor to monitor: Comparing the difference of antral electromyography index between rabbits in ovariectomized group and rabbits in sham operating group; Observing the dose-response relationship between doses of estradiol (0,0.1,0.15,0.2,0.25 and 0.3 mg/kg). To construct the mathematical model, and to analyze the dose-response relationship and mechanism of action. Rresults The expression of estrogen receptor in the antral wall of the stomach was negative. The activity index of gastric antrum myoelectric activity was significantly decreased after ovarian ablation (P<0.01). With the increase of estradiol dose, the activity index of gastric antrum muscle increased and then decreased. Analysis of variance showed that the difference of antral electromyographic activity index between adjacent groups was significant (P<0.05) or extremely significant (P<0.01). Taking the estradiol dose as the indepe ndent variable x, the antral electromyography activity index was the dependent variable y, and the fitting wass obtained: y=2.80 + 5.65 × exp{ -0.5 × ［(x-0.159)/0.038 ］2}. It was a Gaussian function (R=0.9884, P>0.05), which indicated that the regulation of estradiol on gastric antral smooth muscle was based on the intrinsic regulation mechanism of gastric antrum itself, and had a great influence on its internal regulation mechanism. Conclusion The above results indicate that there is no estrogen receptor on the gastric antrum. and the mathematical model of the dose-effect relationship of estradiol on the electrical activity index of gastric antrum in our study accords with the characteristics of Gaussian function model, which indicates that estradiol promotes the activity of gastric antral smooth muscle and is based on the stomach. It shows indirect realization of the internal regulation mechanism of sinus myoelectric activity.

Key words: Estradiol, Myoelectric activity index, Gastric antrum, Mathematical modeling, Immunofluorescence, Female rabbit

CLC Number:

Q424

ZHAO Jian-shuai ZHANG Liu-ning ZHANG Ying-li ZHANG Jin DU Yi-nan GUO Ya-ru LI Xiang WU Yong-jie XU Yong-ping. Mathematical model construction and analysis of the effect of estradiol on the electrical activity of gastric antrum in female rabbits[J]. Acta Anatomica Sinica, 2020, 51(3): 352-360.

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