Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat

doi:10.16098/j.issn.0529-1356.2015.04.008

Abstract

Abstract:

Objective To investigate different expression and its significance of nanog protein in multiple organs fibroblasts of neonatal rats, including heart, liver, spleen, lung, kidney and skin. Methods The expresssion and co-expression of nanog and vimentin in fibroblasts were detected in multiple organs by immunohistochemistry and immunofluorescence. Fibroblasts in multiple organs cultured in vitrowere labeled with nanog and vimentin by immunofluorescence. Results Nanog and vimentin were detected in six organs in neonatal rats respectively. Co-expression rates of these two proteins were（10.07±0.77）% in heart,（9.97±3.70）% in liver,（11.23±2.98）% in spleen,（15.07±2.87）% in lung,（11.75±2.76）% in kidney and（16.14±1.28）% in skin. So co-expression rates of skin and lung were higher than that of other four organs（P<0.05）. Conclusion Fibroblasts in multiple organs which can express nanog may have stem cell-like characters and fibroblasts in skin and lung of neonatal rats have more potential for organ repair.

Key words: Nanog, Vimentin, Fibroblast, Immunohistochemistry, Immunofluorescence, Neonatal rat

LU Zhao-hui CHANG Yu-qiao GUO Zhi-kun* ZHAO Guo-an LI Qiong LI Ci-xia. Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat[J]. AAS, 2015, 46(4): 488-494.

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