Age-related changes of neural stem cells from the subventricular zone of aged mice

doi:10.16098/j.issn.0529-1356.2016.05.001

Abstract

Abstract:

Objective To investigate the biological changes of neural stem cells (NSCs) of mice from subventricular zone (SVZ) during aging. Methods NSCs were isolated, cultured and identified from 2 month (young) and 28 month (aged) old mice respectively, with 10 mice in each group. The proliferation ability of NSCs was determined by BrdU staining; cellular senescence was detected by senescence-associated-β-galactosidase (SA-β-gal) staining; ROS level was determined by ROS kit; expression of cyclin D1, p16, p19 protein in NSCs was tested by Western blotting. The thickness of SVZ between the two groups was detected by nestin/Sox2 staining.Results Positive expressions of neural stem cell marker nestin and Sox2 were identified in NSCs of the young and the aged mice. Aged NSCs exhibited a decreased percentage of BrdU positive cells, increased SA-β-gal activity, elevated intracellular reactive oxygen species level, decreased pool size in the SVZ zone, and there were significant differences (P<0.05). Additionally, at the molecular level, aged NSCs exhibited a decrease of cyclin D1 and an increase of p16 and p19.Conclusion Aged NSCs demonstrate aging changes which may play an important role in brain aging.

Key words: Neural stem cell, Aging, Western blotting, Mouse

DONG Chuan-ming JIN Guo-hua. Age-related changes of neural stem cells from the subventricular zone of aged mice[J]. Acta Anatomica Sinica, 2016, 47(5): 577-582.

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