Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (1): 3-9.doi: 10.16098/j.issn.0529-1356.2024.01.001

• Neurobiology •     Next Articles

Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia

WEI  Xi-xi1,2  LI  Chao-hong1,2  ZHAO  Chen-lu1,2  TANG  Jia-ping1,2  LIU  Yu-zhen1,2*    

  1. 1. Department of Respiratory and Critical Care Medicine; 2. Life Science Research Center, the First Affiliated Hospital of Xinxiang Medical University, He’nan Weihui 453100, China
  • Received:2022-10-31 Revised:2022-12-12 Online:2024-02-06 Published:2024-02-06
  • Contact: LIU Yu-zhen E-mail:yuzhenliu@xxmu.edu.com

Abstract:

Objective  To observe the expression and localization of group Ⅰ metabotropic glutamate receptors (mGluR1/5) in rat superior cervical ganglion (SCG) and the effect of chronic intermittent hypoxia (CIH) on mGluR1/5 protein level.   Methods  Twelve male SD rats were randomly divided into control group(Ctrl)and CIH group(CIH), 6 rats in each group. After 6 weeks of modeling, the effect of CIH on mGluR1/5 protein level was detected by Western blotting, the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining.     Results  mGluR1/5 was expressed in rat SCG. mGluR1 was distributed in neurons and small intensely fluorescent (SIF) cells, but not in satellite glial cells (SGCs), nerve fibers and blood vessels, whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons, but not in SGCs, SIF cells and blood vessels. CIH increased the protein levels of mGluR1/5 (P<0.01) in rat SCG.   Conclusion  Both mGluR1 and mGluR5 are expressed in the rat SCG, but their distribution are different, and the increased protein levels of both may be involved in CIH-induced hypertension.

Key words:

Group Ⅰ metabotropic glutamate receptor, Superior cervical ganglion, Chronic intermittent hypoxia, Hypertension, Western blotting, Rat

CLC Number: