Abstract:

Objective To explore an optimal staining method for observation of mouse kidney juxtaglomerullar cells. Methods Kidney tissues were harvested from 20 mice and prepared as paraffin sections. The sections were randomly divided to 4 groups for 4 different stainings. HE, PAS, modification crystal violet and immunohistochemistry staining, were used for the renal juxtaglomerular cellular secretive granula. Results Neither HE nor PAS methods was able to demonstrate the epithelium-like juxaglomerular cells. Both modification crystal violet method and immunohitochemistry staining methods demonstrated the epithelium-like juxaglomerular cells containing secretive granula. Conclusion Although both modification crystal violet and immunohistochemistry staining methods are able to show better staining result, the modification crystal violet method is more practical than the immunohistochemistry staining method , because of its convenience, easiness, low-cost and stability.

Key words: Renal juxtaglomerular cell, HE staining, Periodic acid-Schiff staining, Modification crystal violet staining, Immunohistochemistry, Mouse