Acta Anatomica Sinica ›› 2017, Vol. 48 ›› Issue (5): 550-555.doi: 10.16098/j.issn.0529-1356.2017.05.009

• Cancer Biology • Previous Articles     Next Articles

Low microRNA-133a level in gastric juice forebodes unfavorable progression and prognosis of gastric cancer

SHAO Juan1,2 KONG Gui-mei1 SHI Ming-yi1 BO Ping1*   

  1. 1. Department of Digestive Diseases Research, Medical College of Yangzhou University, Jiangsu Yangzhou 225001, China; 2.Sanitary Corps of Yangzhou City Detachment, Jiangsu Provincial Corps of Chinese People’s Armed Police Force, Jiangsu Yangzhou 225003, China
  • Received:2017-04-14 Revised:2017-07-06 Online:2017-10-06 Published:2017-10-06
  • Contact: BO Ping E-mail:boping@yzu.edu.cn

Abstract:

Objective We previously reported that the microRNA-133a (MiR-133a) levels in gastric juice and carcinoma tissues of patients with gastric cancer were significantly down-regulated, the current research surveyed whether low MiR-133a levels in gastric juice are related to the progression and prognosis of gastric cancer. Methods The gastric juice and mucosa samples were collected from an independent set of 236 masked pattern: 34 from healthy donors and 202 from patients with gastric cancer. In addition, cell invasion and migration abilities were observed with Transwell invasion assay and wound healing migration assay.
Results MiR-133a levels in gastric juice and carcinoma tissues of patients with gastric cancer were significantly downregulated, and associated with advanced tumor grade, T stage and presence of metastasis. MiR-133a significantly inhibited the invasion and migration abilities of gastric cancer cells. The low MiR-133a expression in gastric juice was taken as a poor prognostic marker of both overall and disease-free survival in patients with gastric cancer. Conclusion These findings justify that low MiR-133a expression in gastric juice strongly associates with advanced tumor progression and unfavorable clinical outcome of patients with gastric cancer, and might be used as a useful tool to estimating progression and prognosis of this disease.

Key words: MicroRNA-133a, Gastric juice, Gastric cancer, Transwell test, Scratch test, Human