Effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 by targeting of cyclic adenosine monophophate responsive element binding protein 1

doi:10.16098/j.issn.0529-1356.2017.06.013

Abstract

Abstract:

Objective To investigate the effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 and to elucidate its action mechanisms. Methods miR-182 mimics or inhibitor were transfected into A375 cells by lipofectamine package, MTT was applied to detect the viability of A375 cells, transwell chamber assay was used to measure the invasion of A375 cells, the expression levels of cyclic adenosine monophosphate responsive element binding protein 1(CREB1) mRNA and protein were detected by Real-time PCR and Western blotting respectively. Results Compared with the untreated group, miR-182 silence significantly increased the proliferation and invasion ability of A375 cells and obviously decreased the expression levels of CREB1 mRNA and protein, P<0.01, but with opposite role for miR-182 overexpression. Conclusion The reinforcing effect of miR-182 silence on the proliferation and invasion of A375 cells may be related to the down-regulation of CREB1.

Key words: miR-182, Proliferation, Invasion, Cyclic adenosine monophosphate responsive element binding protein 1, Transfect, A375 cell, Real-time PCR, Human

WANG Li-na WANG Kai-bo ZHUANG Yong-can WANG Hong-lan. Effects of miR-182 on the proliferation and invasion of human melanoma cell line A375 by targeting of cyclic adenosine monophophate responsive element binding protein 1[J]. Acta Anatomica Sinica, 2017, 48(6): 710-714.

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