Expression characteristics and significance of cathepsin D in the initiation and progression of mouse lung adenocarcinoma

doi:10.16098/j.issn.0529-1356.2018.01.009

Abstract

Abstract:

Objective To investigate the expression characteristics and significance of cathepsin D(CTSD) in the initiation and progression of mouse lung adenocarcinoma.Methods We used urethane intraperitoneal injection to establish lung adenocarcinoma model in the BALB/c mouse. Differentially expressed proteins were identified using isobaric tag for relative and absolute quantitation(iTRAQ)-based quantitative proteomic technology in tumor group compared with control group. Cathepsin D expression characteristics were detected in different time points by immunohistochemical and Western blotting analysis. Results The expression of cathepsin D was significantly increased in the early-stage lung adenocarcinoma tissue compared with the control group. Immonohistochemical and Western blotting analysis observed that following tumor progression, cathepsin D expression was up-regulated. At the late-stage of lung adenocarcinoma, cathepsin D expression was 13.7-fold significantly higher than that in the control group and showed different molecular weight isoform of cathepsin D in the lung tissue of mouse with liver metastasis. Conclusion Cathepsin D is a critical protein in lung carcinogenesis. Aberrant expression and modification of cathepsin D may play an important role in the occurrence, development and metastasis of lung adenocarcinoma. Cathepsin D may have the potential to become biomarkers for early diagnosis of lung adenocarcinoma.

Key words: CathepsinD, Lung adenocarcinoma model, iTRAQ-based quantitative analysis, Immunohistochemistry, Mouse

ZHANG Hui-juan ZHENG Xiao-wei QIAO Ling CHANG Wen-jing LU Feng JIA Cai-yun. Expression characteristics and significance of cathepsin D in the initiation and progression of mouse lung adenocarcinoma[J]. Acta Anatomica Sinica, 2018, 49(1): 56-62.

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