Distribution and expression changes of the vesicular glutamate transporter 1 and vesicular glutamate transporter 2 immunopositive fibers and terminals in the postnatal development of the spinal cord of rat

doi:10.16098/j.issn.0529-1356.2018.03.003

Abstract

Abstract:

Objective To explore different distribution and expression changes of vesicular glutamate transporter 1(VGLUT1) and VGLUT2 immunopositive (-ip) fibers and terminals in the spinal cord of postnatal 0 day (P0)-22 days (P22) rats. Methods Immunohistochemical staining was performed for the VGLUT1 and VGLUT2 in the cervical and lumber enlargements of P0-P22 rats. Results VGLUT1-and VGLUT2-ip fibers and terminals were observed in the cervical and lumber enlargements of the spinal cord in the postnatal P0-P22 rats but not soma. These fibers and termianls showed a typical complementary distribution pattern, especially in the posterior horn of the spinal cord. On P0, VGLUT1-ip fibers and terminals were mainly observed in the laminae Ⅲ-Ⅴ of the cervical and lumber enlargements, with a weak expression in the intermediate gray and anterior horn. From P3, the expression of VGLUT1 was increased in the laminae Ⅲ-Ⅴ and extended to the lateral, and the VGLUT1-ip profiles were detected in the basal part of posterior horn (lamina Ⅵ) and the lateral part of the anterior horn (lamina Ⅳ). An obvious trend of the belt distribution from the dorsomedial to ventrolateral side was observed. It was more obvious at P7, and expanded gradually to the medial and lateral side of spinal grey with development. On P22, VGLUT1-ip profiles were observed on almost whole spinal gray, except for a week expression in the lamina Ⅱ. On the contrary, VGLUT2-ip fibers and terminals were observed to distribute densely in laminae I-Ⅱ and the margins of the anterior horn from P0. In the other areas of the spinal cord, medium density of VGLUT2-ip fibers and terminals were distributed much more evenly. With the development, the distribution pattern of VGLUT2-ip fibers and terminals were not changed greatly, although its density was gradually increased. On the other hand, in the posterior cord of the spinal white matter, the developmental process of the VGLUT1 positive corticospinal posterior tract gradually went down from the cervical (P3) to the lumbar cord (P7). Conclusion The distribution of VGLUT1-and VGLUT2-ip fibers and terminals is largely different in the postnatal developing cervical and lumber spinal cord of rats, showing an obvious complementary pattern. This may be important for us to understand the difference of their functional involvement during the postnatal development of the spinal cord.

Key words:

Postnatal development, Spinal cord, Vesicular glutamate transporter 1, Vesicular glutamate transporter 2, Immunohistochemistry, Rat

LI Jing CHEN Tao LI Jin-lian.

Distribution and expression changes of the vesicular glutamate transporter 1 and vesicular glutamate transporter 2 immunopositive fibers and terminals in the postnatal development of the spinal cord of rat

[J]. Acta Anatomica Sinica, 2018, 49(3): 288-293.

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