Persistent hyperglycemia induced by chronic restraint stress in rat is associated with nucleus tractus solitarius injury

doi:10.16098/j.issn.0529-1356.2019.04.004

Abstract

Abstract:

Objective To investigate the role of anterior part of commissural subnucleus of nucleus tractus solitarius (acNTS) injury in insulin-resistant hyperglycemia during chronic restraint stress (CRS). Methods We produced the CRS models (n=20， a 7-day restraint followed by a 3-day free moving procedure for 40 days) in rats, and detected the parameters related to glucose metabolism. Results The CRS induced a moderate (not higher than 11 mmol/L) and irreversible insulinresistant hyperglycemia in about 1/3 (n=7) of the individuals. CRS-hyperglycemic rats showed a condensed staining of acNTS neurons, and Caspase-3 immunostaining and TUNEL also showed positive, indicating apoptotic changes of acNTS neurons. After acNTS mechanical damage (n=6), the blood glucose level rised gradually, which also led to insulin-resistant hyperglycemia. The characteristics of hyperinsulinemia, increased islet volume, and serum corticosterone levels in acNTS mice were consistent with those of CRS mice. Conclusion The result indicates that during CRS, injury (apoptosis) of glucose-sensitive acNTS neurons causes dysregulation of blood glucose. Restraint stress model has value as a potential application in the study of stress-induced hyperglycemia.

Key words:

Chronic restraint stress, Insulin-resistant hyperglycemia, Neuron injury, Nucleus tractus solitaries, Enzyme-linked immunosorbent assay, Rat

BI Wen-jie ZHENG Xiang. Persistent hyperglycemia induced by chronic restraint stress in rat is associated with nucleus tractus solitarius injury[J]. Acta Anatomica Sinica, 2019, 50(4): 423-430.

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