Expression of Bcl-2 and Bax after cerebral ischemia-reperfusion injury in rats with hyperlipemia and the effect of scutellarin

doi:10.16098/j.issn.0529-1356.2019.04.005

Abstract

Abstract:

Objective To investigate the effect of scutellarin after cerebral ischemia-reperfusion injury in rats with hyperlipemia,by observing the expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2 assaciated X protein (Bax) in cerebral cortex with hyperlipemia. Methods After the rat model of hyperlipidemia was established, the focal cerebral ischemia-reperfusion model in hyperlipemia rats was established with thread embolism of the middle cerebral artery. The neurobehavioral score was used to observe the symptoms of neurobehavioral injury. 2,3,5 -triphenyl tetrazolium chloride (TTC）staining was used to observe the cerebral infarct volume. HE staining was used to observe the pathological changes of brain tissue. Immunohistochemistry and Western blotting were used to observe the expressions of Bcl-2 and Bax. Results Compared with the sham-operation group, the neurobehavioral scores,cerebral infarction volum and pathologic damage were significantly increased,the expressions of Bcl-2 was significantly lower, the expressions of Bax was significantly increased in ischemiareperfusion group(P<0.05). Compared with the saline group, the neurobehavioral scores, cerebral infarction volum and pathologic damage were significantly decreased,the expression of Bcl-2 was significantly increased, the expression of Bax was significantly decreased in scutellarin treatment group (P<0.05). Conclusion In cerebral ischemia-reperfusion injury rats with hyperlipemia, scutellarin can alleviate cerebral ischemia-reperfusion injury by promoting the expression of Bcl-2 and inhibiting the expression of Bax.

Key words:

Hyperlipemia, Cerebral ischemia-reperfusion injury, B-cell lymphoma-2, Bcl-2 assaciated X protein, Scutellarin, Western blotting, Rat

YANG Ying-chun WANG Rui-fang CHEN Xin-ji ZHANG Xiao-liang GAO Sai-hong REN Zhan-chuan. Expression of Bcl-2 and Bax after cerebral ischemia-reperfusion injury in rats with hyperlipemia and the effect of scutellarin[J]. Acta Anatomica Sinica, 2019, 50(4): 431-437.

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