Objective To investigate the changes of intestinal microbes in rotenone-induced Parkinson’s disease (PD) mice based on 16S rRNA gene sequencing.   Methods Fourteen 8-weekold male C57BL/6J mice were randomly divided into two groups: 6 mice in the control group and 8 mice in the model group. The model mice were injected subcutaneously with rotenone (3 mg/kg) for 5 weeks, and the body weight was measured once a week. After 5 weeks, behavioral tests were performed, including the rotating rod test and the open field test. The contents of the tract were used for intestinal microbial detection analysis.  Results After 5 weeks of rotenone treatment, the weight of PD mice was significantly lower than that of the control mice（P<0.05）. The movement time of the PD model mice in the rotating rod（P<0.0001）and the total distance of movement in the open field（P<0.05）were significantly lower than that of the control mice. In addition, the intestinal microbial diversity of the PD model mice did not change（P>0.05）,but the microbial species showed significant differences. Among them, the PD mice showed a significant decrease in the intestinal  Turicibacter（P<0.01）, a significant increase in norank_f_Lachnospiraceae（P<0.01）, a significant decrease in norank_f_ Erysipelotrichacea e（P<0.01）, and a significant increase in Lachnoclostridium（P<0.01）.  Conclusion Intestinal microbes in PD mice are disordered, and these intestinal flora may be involved in the development of dyskinesia in PD mice.