Acta Anatomica Sinica

Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (1): 10-16.doi: 10.16098/j.issn.0529-1356.2024.01.002

• Neurobiology • Previous Articles     Next Articles

Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle

ZHENG  Li-ping1  LIU  Xia1* DU  Xiao-hua2  WU  Ya-juan2  LIU  Shan-shan2   

  1. 1.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2022-08-23 Revised:2022-11-28 Online:2024-02-06 Published:2024-02-06
  • Contact: LIU Xia E-mail:liux@gsau.edu.cn

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Abstract:

Objective  To clarify the expression and distribution of brain-derived neurotrophic factor (BDNF) in the cerebrum of plateau yaks and cattle, and to explore the relationship between BDNF function and the adaptability of altitude hypoxia.    Methods  Five yaks and five cattles were selected.The content and distribution of BDNF in frontal lobe, temporal lobe, parietal lobe, occipital lobe, cerebrum white matter and hippocampus of yak and cattle were analyzed by Real-time PCR, Western blotting and Immunohistochemistry.   Results  Real-time PCR result  showed that BDNF mRNA expression in the cerebrum of yaks and cattles was highest in temporal cortex, followed by hippocampus, parietal cortex, occipital cortex and frontal cortex, and lowest in white matter.  Western blotting results showed that the content of BDNF protein in the cerebrum of yaks was the highest in temporal cortex,followed by hippocampus. The content of BDNF protein in other tissues was parietal cortex, frontal cortex and cerebrum white matter, and the content of BDNF protein was the lowest in occipital cortex. The content of BDNF protein intlecerebrum of cattles was the highest in the temporal cortex, followed by the hippocampus. The content of BDNF protein in other tissues was parietal cortex, occipital cortex and frontal cortex in descending order, and the protein content in cerebrum white matter was the lowest.  Immunohistochemical results showed that the positive expression of BDNF protein in the cerebrum of yaks and cattles was basically similar, mainly distributed in the granulosa cells and glial cells in the frontal cortex, temporal cortex, parietal cortex and occipital cortex, glial cells in cerebrum white matter, pyramidal cell layer and polyform cell layer in the hippocampus. There was the small amount of distribution in Martinotti cells and the molecular layer of hippocampus in the cerebral cortex.   Conclusion  BDNF mRNA and protein are distributed and expressed in different brain regions of yaks and cattles, but the expression level different, which is speculated to be closely related to the specific functions of different cerebrum regions. The expression level of the cerebrum of yak is higher than that of cattle except occipital cortex, suggesting that it is related to the altitude hypoxic environment. BDNF may play an important role in enhancing hypoxic tolerance and protecting internal environmental homeostasis in the process of animal adaptation to hypoxic environment.

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