Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

Abstract

Abstract: Objective To observe the Bmi-1 protein expression in esophageal epithelial tissues ,and to explore the relationship between the expression pattern of Bmi-1 and the development of esophageal epithelial tissues. Methods Immunohistochemistry was used to measure the expression of Bmi-1 in esophageal epithelial tissues of 120 cases mouse in different development time,15 cases human embryos, 18 cases normal adult human and 17 cases human esophageal squamous cell carcinoma (ESCC), then the level of Bmi-1 protein expression in these samples were analyzed by image analysis software, and the statistical analyses were applied to test the relationship between the expression of Bmi-1 and each group. Results Bmi-1 was found to expression in every time of development, but the differences were present in the protein expression level (P＜0.05). Expression of Bmi-1 protein was higher at embryo stage than that after birth both in mice and human. Compared with esophageal tissue of normal adult human, Bmi-1 overexpressed both in human esophageal squamous cell carcinoma and human embryo(P＜0.01). Conclusions The expression of Bmi-1 closely was related with the development of esophageal epithelium.Furthermore,there were a potential significance to detection of the Bmi-1 expression for the research in the developmental biology of esophageal epithelium and the cytobiology of esophageal cancer.

Key words: Bmi-1, esophageal epithelium , development, Immunohistochemistry

References

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