解剖学报

›› 2007, Vol. 38 ›› Issue (3): 278-281.doi:

• 论著 • 上一篇    下一篇

侧脑室注射链脲佐菌素对大鼠海马突触相关蛋白PSD-95和Shank 1表达的影响

陆珊1，2; 雷亚平1，5; 崔艳君3 ;王蓬文1，3*; 王蓉4 ; 盛树力4 ; 田金洲1   

  1. 1北京中医药大学东直门医院 北京 100700；2中关村医院，北京 100080；3首都医科大学基础医学院病理学教研室，北京 100069；4教育部神经变性病学重点实验室，北京 100053；5房山中医院病理科，北京 102400
  • 收稿日期:2006-04-13 修回日期:2006-12-25 出版日期:2007-06-06
  • 通讯作者: 王蓬文

EFFECTS OF INTRACEREBROVENTRICULAR ADMINISTRATION OF STREPTOZOTOCIN ON THE EXPRESSION OF PSD-95 AND Shank 1 IN RATS

  1. 1Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700; 2Zhongguanchun Hospital, Beijing 100080;3Department of Pathology, College of Basic Medical Sciences, Capital Medical University, Beijing 100069;4Key Laboratory of Ministry of Education for Neurodegenerative Diseases, Beijing 100053;5Fangshan Chinese Medicine Hospital, Beijing 102400,China
  • Received:2006-04-13 Revised:2006-12-25 Online:2007-06-06
  • Contact: WANG Peng-wen

关键词: 链脲佐菌素, 侧脑室注射, PSD-95, Shank1, 免疫组织化学, 免疫印迹, 大鼠

Abstract: Objective To investigate the effects of intracerebroventricular（ICV） administration of streptozotocin (STZ) on expression of PSD-95 and Shank1 in rat hippocampus. Methods A total of 30 rats were randomly divided into normal control group and model group. The rats in the model group received intracerebroventricular injection of STZ bilaterally with a dose of 3mg/kg and the injection was repeated on the third day. The rats in normal control group received the same treatment as the first group except for STZ injection. After 21 days, the brains of the rats were taken, frozen sections of brain were prepared, and then PSD-95 and Shank1 were stained with immunohistochemistry. Furthermore, the contents of PSD-95 and Shank1 were detected by Western blotting. Results After ICV injection of STZ, the expression of PSD-95 and Shank1 positive cells and the contents of PSD-95 and Shank1 in hippocampus both decreased. Conclusion ICV injection of STZ can lead to the decrease of the expression of PSD-95 and Shank1, indicating it may damage the neuron’s synaptic signal

Key words: Streptozotocin, Intracerebroventricular injection, PSD-95, Shank1, Immunohistochemistry, Western blotting, Rat

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