解剖学报

解剖学报 ›› 2023, Vol. 54 ›› Issue (6): 716-721.doi: 10.16098/j.issn.0529-1356.2023.06.014

• 组织学胚胎学发育生物学 • 上一篇    下一篇

高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制

 许亚平1 余悦欣1 陈金福2 王柯柯1 郭志坤1,2*   

  1. 1. 郑州市第七人民医院河南省心脏重构与移植重点实验室，郑州 450000; 2. 新乡医学院河南省医用组织再生重点实验室，河南 新乡 453003
  • 收稿日期:2022-08-25 修回日期:2023-02-01 出版日期:2023-12-06 发布日期:2023-12-06
  • 通讯作者: 郭志坤 E-mail:gzk@xxmu.edu.cn

Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats

XU Ya-ping1  YU Yue-xin1  CHEN Jin-fu2  WANG Ke-ke1  GUO Zhikun1,2*   

  1. 1.He’nan Provincial Key Laboratory of Heart Reconstruction and Transplantation, Zhengzhou Seventh People’s Hospital, Zhengzhou 450000, China;  2.He’nan Provincial Key Laboratory of Tissue Regeneration, Xinxiang Medical University, He’nan Xinxiang 453003, China

  • Received:2022-08-25 Revised:2023-02-01 Online:2023-12-06 Published:2023-12-06
  • Contact: GUO ZHIKUN E-mail:gzk@xxmu.edu.cn

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摘要：

目的 探讨高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制。  方法 青年SD大鼠(24周)和高龄SD大鼠(104周)各5只,小动物超声检测心功能，采用改良Weigert弹力纤维染色、免疫组织化学、免疫荧光和Western blotting，分别检测心室肌Ⅰ型和Ⅲ型胶原纤维及蛋白、弹性纤维及蛋白、基质金属蛋白酶2 (MMP-2)、基质金属蛋白酶9(MMP-9)和基质金属蛋白酶抑制剂2(TIMP-2)的表达变化。  结果 高龄大鼠心室肌间质Ⅰ型和Ⅲ型胶原蛋白丰富，包绕心肌细胞周围，与青年大鼠相比，高龄大鼠心室肌间质胶原蛋白含量明显增多(P<0.05)。高龄大鼠心室肌间质弹性纤维分布广泛，与青年大鼠比较，高龄大鼠心室肌间质弹性纤维数量和弹性蛋白水平均明显减少(P<0.05)。高龄大鼠心室肌MMP-2和MMP-9水平增高，且与弹性蛋白水平呈相互消长关系。高龄大鼠心室肌TIMP-2水平随年龄增加而降低。  结论 高龄大鼠心室肌间质胶原纤维数量和弹性纤维数量存在相互消长的关系。随年龄的增长大鼠心室肌间质TIMP-2和弹性纤维含量减少, 同时胶原纤维与弹性纤维比例失调。

关键词: 基质金属蛋白酶2, 基质金属蛋白酶9, 基质金属蛋白酶抑制剂2, 胶原纤维, 弹性纤维, 心室肌, 免疫荧光, 免疫印迹法, 大鼠

Abstract:

Objective  To investigate the structural distribution features and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats.  Methods Five young SD rats (24 weeks) and five old SD rats (104 weeks) were used，and their cardiac function was examined by echocardiography. Modified Weigert elastic fiber staining, immunohistochemistry, immunofluorescence and Western blotting techniques were used to detect the expression changes of type I and Ⅲ collagen fibers and their proteins, elastic fibers and their proteins, matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinase 2 (TIMP-2), respectively.    Results The type I and type Ⅲ collagen in the ventricular interstitium of aged rats was very sufficient and wrapped around the cardiomyocytes. Compared with the young rats, the content of collagen protein in the ventricular interstitium of the aged rats significantly increased (P<0.05). Elastic fibers in the ventricular interstitium of the aged rats were and widely distributed. Compared with the young rats, the number of elastic fibers and the level of elastin in the ventricular interstitium of the aged rats significantly decreased (P<0.05), and the expression levels of MMP-2 and MMP-9 in ventricular muscle of aged rats increased, and they were correlated with the level of elastin. The level of TIMP-2 in ventricular muscle of aged rats decreased with age.   Conclusion The number of collagen fibers and elastic fibers in ventricular interstitium of aged rats is fluctuated with each other. With the increase of age, the contents of TIMP-2 and elastic fibers in the ventricular interstitium gradually decreased, and the ratio of collagen fibers to elastic fibers is out of balance.

Key words: Matrix metalloproteinase 2, Matrix metalloproteinase 9, Tissue inhibitor of metalloproteinase 2, Collagen fiber, Elastic fiber, Ventricular muscle, Immunofluorescence, Western blotting, Rat

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