AAS ›› 2016, Vol. 47 ›› Issue (4): 469-475.doi: 10.16098/j.issn.0529-1356.2016.04.06

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Effect of amyloid beta-peptide25-35 neurotoxicity on cytoskeletons of  PC12 cells

CAO Jing-jing FAN Wen-juan SHI Zhen-yu YAN Ming-chao LIU Hong-liang WANG Lai DENG Jin-bo*   

  1. Institute of Neurobiology, He’nan University, He’nan Kaifeng 475004, China
  • Received:2016-01-12 Revised:2016-04-21 Online:2016-08-06 Published:2016-08-06
  • Contact: DENG Jin-bo E-mail:610624718@qq.com

Abstract:

Objective To establish the cell model of Alzheimer’s disease (AD) and investigate the amyloid beta-peptides 25-35(Aβ 25-35) neurotoxicity to cytoskeleton.
Methods PC12 cells were cultured and treated with Aβ 25-35, and cell survival was analyzed by MTT assay. Cell apoptosis was visualized with DAPI staining and TUNEL method. Immunocytochemistry and phalloidin staining were used to label cytoskeletons in PC12 cells. Results Aβ 25-35 obviously induced the PC12 cells death and lead to PC12 cells apoptosis with dose dependency (P<0.05). Aβ 25-35 gave rise to the disintegration of cytoskeletons with dose dependency (P<0.05). Conclusion PC12 cell cytoskeletons are sensitive to Aβ 25-35 neurotoxicity. The disintegration of cytoskeleton probably is the important pathological alteration in AD, and Aβ is a key molecule for AD pathogenesis.

Key words: Alzheimer’s disease, Amyloid beta-peptides 25-35, Cytoskeleton, Terminal UTP nick end labeling, Immunohistochemistry, PC12 cell