AAS ›› 2015, Vol. 46 ›› Issue (1): 38-43.doi: 10.16098/j.issn.0529-1356.2015.01.007

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Neuroprotective effect of  Xuebijing following the brain injury in the rat after cardiopulmonary resuscitation

WU Yi1 CHENG Xiang2 YI Xin2 JI Da-feng2 HAN Xiao2 HUANG Zhong-wei 3*   

  1. 1. Intensive Care Unit of Nantong Tumor Hospital; 2.Department of Human Anatomy,Nantong University; 3.Emergency Department of Nantong Unuiversity Affilated Hospital,Jiangsu Nantong 226001,China
  • Received:2014-06-16 Revised:2014-10-31 Online:2015-02-06 Published:2015-02-06
  • Contact: HUANG Zhong-wei E-mail:hzw889@163.com

Abstract:

Objective To explore the effect of Chinese medicine Xuebijing against post cardiopulmonary-resuscitatied brain injury in rats and the related mechanisms involved.
Methods The rat model of cardiopulmonary-resuscitatied brain injury was established according to the model of Utstein. The rats were randomly divided into 3 groups: Sham group, Xuebijing group and physiological saline group. After the rat model of cardiopulmonary-resuscitatied brain injury had been established in Xuebijing group and physiological saline group, Xuebijing or physiological saline were injected into cardiopulmonary resuscitation model rats by intraperitoneal injection respectively. At 10 days after establishment rat model of cardiopulmonary resuscitatied brain injury, the continuous rat brain frozen sections were acquired to reconstruct theion rat brain by 3D-Doctor, and the volumes of the damaged region in the rat brain were calculated by 3D-Doctor. Frozen sections of brain damaged tissues were subjected to detect apoptotic cells by using the terminal deoxynucleotide transferase dUTP nick end labeling(TUNEL) assay. Results The rat model of cardiopulmonary-resuscitatied brain injury was established successfully,with 50% of thewhich success ratepercentage was 50%. Rat brains in physiological saline group and Xuebijing group were reconstructed successfully by 3D-Doctor, which showed the volume of infarction in Xuebijin group reduced significantly compared with that of the physiological saline group. TUNEL assay showed that apoptotic reaction occurred in the cortex around the injury site. After treatment with Xuebijing, the apoptosis index (AI) was significantly decreased compared with that of physiological saline group. Conclusion For cardiopulmonary resuscitatied brain damage in rats, intraperitoneal injection of Xuebijing can reduce the rat brain cortex injured area and infarct size. Xuebijing can also inhibit neural cells apoptosis around damaged cortex, which has the neuroprotective effect on brain injury in the rat after cardiopulmonary resuscitation.

Key words: Cardiopulmonary-resuscitatied brain injury, Xuebijing, Neuroprotection, 3D reconstruction, Rat