解剖学报

解剖学报 ›› 2015, Vol. ›› Issue (3): 400-404.doi: 10.16098/j.issn.0529-1356.2015.03.019

• 组织学胚胎学发育生物学 • 上一篇    下一篇

细胞色素P450ⅡE1在肠道血吸虫病并结直肠癌患者肠黏膜中的表达

李钢琴 张宇星* 龚立刚 袁修学 程聪 李盈 黄元   

  1. 武汉市第八医院消化内镜中心，武汉 430010
  • 收稿日期:2015-01-15 修回日期:2015-03-04 出版日期:2015-06-06 发布日期:2015-06-06
  • 通讯作者: 张宇星 E-mail:84669442@qq.com

Expression of cytochrome P450Ⅱ E1 in colorectal carcinoma complicated with intestinal schistosomiasis

LI Gang-qin ZHANG Yu-xing*GONG Li-gang YUAN Xiu-xue CHENG Cong LI Ying HUANG Yuan   

  1. Center for Digestive Endoscopy, the Eighth Hospital (Colorectal Hospital)of Wuhan, Wuhan 430010,China
  • Received:2015-01-15 Revised:2015-03-04 Online:2015-06-06 Published:2015-06-06
  • Contact: ZHANG Yu-xing E-mail:84669442@qq.com

摘要：

目的 探讨细胞色素P450ⅡE1（CYPⅡE1）的蛋白表达与肠道血吸虫病并结直肠癌之间的相关性及其可能的作用机制。方法 采用免疫组织化学SP法，检测60例肠道血吸虫并结直肠癌癌组织、60例单纯结直肠癌癌组织（无肠道血吸虫感染）、60例单纯肠道血吸虫感染组、50例无血吸虫感染的正常肠道组织中的CYPⅡE1蛋白的表达情况；分析肠道血吸虫病并结直肠癌癌组织中CYPⅡE1的表达与患者的性别、年龄、病理分型、淋巴结转移等临床病理参数的关系。 结果 肠道血吸虫病并结直肠癌组织中CYPⅡE1的表达阳性为73.33%，单纯肠道血吸虫感染组表达阳性为46.67%，60例单纯结直肠癌癌组织（无肠道血吸虫感染）表达阳性为31.67%，而正常组织中的表达为16%。在黏液腺癌中CYPⅡE1蛋白表达明显增高（P＜0.01）。血吸虫并结直肠癌组CYPⅡE1蛋白表达阳性与性别、年龄、肿瘤部位、淋巴结转移无关，与组织分化程度和病理分型有关。逐步回归法进行 Logistic多因素分析结果显示，组织学分型是CYPⅡE1表达阳性的唯一危险因素（OR=11.4，P=0.024）。 结论 CYPⅡE1可能在肠道血吸虫病并结直肠癌的发病机制中有一定的作用。CYPⅡE1的表达与结直肠癌组织的病理分型有关。

Abstract:

Objective To study the relationship between cytochrome P450Ⅱ E1(CYPⅡE1) protein expression of colorectal carcinoma complicated with intestinal schistosomiasis and its possible mechanism. Methods Immunohistochemical SP method was applied to detect the CYPⅡE1 protein expression of 60 cases of intestinal schistosomiasis and colorectal cancer tissue, 60 cases of colorectal cancer tissue without intestinal infection of Schistosoma japonicum，60 cases of intestinal schistosomiasis infection group, and 50 cases of normal intestinal tissue without infection of Schistosoma japonicum. Results The rates of the cytochrome p450Ⅱ E1 immunohistochemical positive staining were 73.33% in colorectal carcinoma complicated with intestinal schistosomiasis group, 46.67% in the intestinal schitosoma japonicum infection group, 31.67% in colorectal cancer tissue without intestinal infection of schistosoma japonicum, 16% in the normal intestinal tissues. The cytochrome p450Ⅱ E1 immunohistochemical positive in colorectal carcinoma complicated with intestinal schistosomiasis group associated without gender, age, tumor location, lymph node metastasis, but with tissue differentiation degree and pathology classification.The stepwise regression method of logistic multiple factor analysis showed that the histological type was the only risk factors for the positive expression of P450Ⅱ E1 （OR=11.4，P=0.024). Conclusion CYPⅡE1 expression is associated with colorectal cancer pathological tissue type. CYP II E1 plays a certain role in the pathogenesis of in colorectal carcinoma complicated with intestinal schistosomiasis.