可溶性晚期糖基化终末产物受体拮抗缺血/再灌注小鼠心肌纤维化

doi:10.16098/j.issn.0529-1356.2021.05.015

解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 772-776.doi: 10.16098/j.issn.0529-1356.2021.05.015

• 组织学胚胎学发育生物学 • 上一篇下一篇

可溶性晚期糖基化终末产物受体拮抗缺血/再灌注小鼠心肌纤维化

曹贤贤¹韩雪洁¹王红霞² 曾翔俊^2* 郭彩霞^1*

1.首都医科大学附属北京同仁医院心血管中心，北京 100073; 2.首都医科大学生理学与病理生理学系，北京 100069

收稿日期:2020-04-05 修回日期:2020-04-15 出版日期:2021-10-06 发布日期:2021-10-06
通讯作者: 曾翔俊;郭彩霞 E-mail:cxgbb@163.com
基金资助:
国家自然科学基金;国家自然科学基金

Soluble receptor for advanced glycation end-products inhibits myocardial fibrosis of ischemia/reperfusion mice

CAO Xian-xian¹ HAN Xue-jie¹ WANG Hong-xia² ZENG Xiang-jun^2* GUO Cai-xia^1*

1.Cardiovascular Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; 2.Department of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, China

Received:2020-04-05 Revised:2020-04-15 Online:2021-10-06 Published:2021-10-06
Contact: ZENG Xiang-jun; GUO Cai-xia E-mail:cxgbb@163.com

摘要/Abstract

摘要：

目的探讨可溶性晚期糖基化终末产物受体（sRAGE）对缺血/再灌注小鼠心脏炎症的影响及其机制。方法利用6～8周龄雄性C57BL/6小鼠构建心肌缺血/再灌注损伤模型（左前降支结扎30 min，再灌注2周），随机分为4组，每组5只C57BL/6小鼠。通过心脏超声检测心功能，HE染色观察炎症细胞浸润情况，马松和天狼星红染色检测心肌纤维化，免疫组织化学染色检测半乳糖凝集素3（galecti-3）表达。结果与假手术组相比，缺血/再灌注组小鼠的心功能减退，心肌组织间有大量炎症细胞浸润，心肌纤维化面积增加，同时，心脏组织中 galectin-3 的表达增多；而给予外源性sRAGE处理后，小鼠的心功能显著改善，炎症细胞浸润减少，纤维化程度减轻，心脏组织中galecti-3的表达也显著减少。结论心肌缺血/再灌注损伤时，sRAGE可能通过抑制galectin-3的表达，减少小鼠心脏炎症细胞浸润，从而减轻心肌纤维化。

关键词: 可溶性晚期糖基化终末产物受体, 缺血/再灌注, 纤维化, 免疫组织化学, 小鼠

Abstract:

Objective To investigate the effect of soluble receptor for advanced glycation end-products (sRAGE) on inflammation in myocardial ischemia/reperfusion(I/R) mice and its mechanism. Methods Myocardial I/R injury model was conducted by left anterior descending ligation for 30 minutes and reperfusion for 2 weeks in male C57BL/6 mice aged 6-8 weeks. The mice were randomly divided into four groups with five C57BL/6 mice in each group. The cardiac function was detected by echocardiography, the inflammatory cells infiltration was observed by HE staining, the myocardial fibrosis was detected by Masson and Sirius red staining, the expression of galectin-3 was detected by immunohistochemical staining. Results Compared with the sham group, the cardiac function decreased, the inflammatory cells infiltrated increased among the myocardial tissue, the percentage of myocardial fibrosis area increased, and the expression of galectin-3 increased in I/R groups. After exogenous sRAGE treatment, the cardiac function of mice was significantly improved, the inflammatory cells infiltration decreased, the myocardial fibrosis area decreased, and the expression of galectin-3 decreased as well. Conclusion sRAGE may reduce inflammatory cells infiltration in mice heart by inhibiting the expression of galectin-3, and then alleviating myocardial fibrosis during myocardial ischemia/reperfusion injury.

Key words: Soluble receptor for advanced glycation end-products, Ischemia/reperfusion, Fibrosis, Immunohistochemistry, Mouse

中图分类号:

曹贤贤韩雪洁王红霞曾翔俊郭彩霞 . 可溶性晚期糖基化终末产物受体拮抗缺血/再灌注小鼠心肌纤维化[J]. 解剖学报, 2021, 52(5): 772-776.

CAO Xian-xian HAN Xue-jie WANG Hong-xia ZENG Xiang-jun GUO Cai-xia. Soluble receptor for advanced glycation end-products inhibits myocardial fibrosis of ischemia/reperfusion mice[J]. Acta Anatomica Sinica, 2021, 52(5): 772-776.

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可溶性晚期糖基化终末产物受体拮抗缺血/再灌注小鼠心肌纤维化

Soluble receptor for advanced glycation end-products inhibits myocardial fibrosis of ischemia/reperfusion mice

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