解剖学报

›› 2011, Vol. 42 ›› Issue (1): 129-132.doi: 10.3969/j.issn.0529-1356.2011.01.024

• 论著 • 上一篇    下一篇

编码精子赤道段蛋白的质粒DNA免疫影响小鼠生育功能

吕正梅1,2;王旻1,3;徐晨1,3*   

  1. 1.Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;2.Department of Histology and Embryology, Anhui Medical University, Hefei 230032, China;3. Shanghai Key Laboratory for Reproductive Medicine, Shanghai 200025, China
  • 收稿日期:2010-02-12 修回日期:2010-04-18 出版日期:2011-01-06
  • 通讯作者: 徐晨

Antifertility effect in mice after vaccination with plasmid DNA encoding mouse sperm equatorial segment protein

  1. 1. 上海交通大学医学院组织学胚胎学教研室，上海 200025; 2.安徽医科大学组织学胚胎学教研室，合肥 230032; 3. 上海市生殖医学重点实验室， 上海 200025
  • Received:2010-02-12 Revised:2010-04-18 Online:2011-01-06
  • Contact: XU Chen1

摘要： P>

关键词: 精子赤道段蛋白, DNA疫苗, 免疫避孕, 双重免疫荧光技术, 反转录-聚合酶链式反应, 小鼠

Abstract: Objective To determine the feasibility of pcDNA3-1/mESP as a potential immunocontraceptive antigen. Methods Eukaryotic expression plasmid pcDNA3-1/mESP, encoding N-terminal of mouse sperm equatorial segment protein (mESP), was constructed. Two groups of mice（EM>n/EM>=8/group） were inoculated with plasmids of pcDNA3-1/mESP and pcDNA3-1 respectively. RT-PCR， ELISA and double immunofluorescence assay were performed to observe pcDNA3-1/mESP expression and immune response in the inoculated mice. Mouse mating test was employed to study fertility of the experimental and control group. Results mESP cDNA positive band was detected in muscle from mice immunized with pcDNA3-1/mESP，which identified the expression of the recombinant plasmid. The pcDNA3-1/mESP induced specific antibody against mESP in the experimental mice. Significant difference of mean litter size between experimental and control groups was observed, while the rate of fertility did not decrease. Conclusion This study indicates the need to strengthen DNA vaccines in order to obtain

Key words: Sperm equatorial segment protein, DNA vaccine, Immunocontraception, Double immunofluorescence technique, RT-PCR, Mouse

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