解剖学报

解剖学报 ›› 2023, Vol. 54 ›› Issue (3): 261-268.doi: 10.16098/j.issn.0529-1356.2023.03.002

• 神经生物学 • 上一篇    下一篇

雌二醇经雌激素受体调控分拣蛋白相关受体A对小鼠海马神经元树突棘密度和突触蛋白表达的影响

张沂洲1,2,3 李莎1,2,3 米世雄1,2,3 左洪春1,2 张倩倩4 李晗琳5 雷梓晗5 张东泽5 崔慧先1,2,3*   

  1. 1.河北医科大学人体解剖学教研室; 2.河北医科大学神经科学中心; 3.河北省神经退行性疾病机制研究重点实验室; 4.河北医科大学精神医学系; 5.河北医科大学临床医学系，石家庄    050017
  • 收稿日期:2022-06-16 修回日期:2022-11-14 出版日期:2023-06-06 发布日期:2023-06-06
  • 通讯作者: 崔慧先 E-mail:cuihxhebmu@126.com
  • 基金资助:
    河北省自然科学基金;河北省高等学校科学技术研究项目;河北医科大学大学生创新性实验计划项目

Effect of estradiol regulating sortilin-related receptor A expression on hippocampal spine density and synaptic protein expression via estrogen receptor of mouse

ZHANG  Yi-zhou1,2,3  LI  Sha1,2,3   MI  Shi-xiong1,2,3  ZUO  Hong-chun1,2  ZHANG  Qian-qian4 LI  Han-lin5   LEI  Zi-han5  ZHANG  Dong-ze5  CUI  Hui-xian1,2,3*   

  1. 1.Department of Anatomy, Hebei Medical University; 2.Neuroscience Research Center, Hebei Medical University; 3.Hebei Key Laboratory of Neurodegenerative      Disease Mechanism;   4.Department of Psychiatry, Hebei Medical University; 5.Clinical Medicine, Hebei Medical University, Shijiazhuang 050017, China
  • Received:2022-06-16 Revised:2022-11-14 Online:2023-06-06 Published:2023-06-06
  • Contact: CUI Hui-xian E-mail:cuihxhebmu@126.com

PDF

摘要：

目的 探讨雌二醇（E2）调控分拣蛋白相关受体A（SorLA）表达对小鼠海马神经元树突棘密度和突触蛋白表达的影响及机制。  方法 32只C57BL/6雌性小鼠随机分为假手术（sham）组、卵巢切除（OVX）组、卵巢切除+E2组（OVX+E2）组、卵巢切除+氟维司琼（Fu）+E2组（OVX+Fu+E2）组，通过Western blotting和免疫组织化学染色检测小鼠海马CA1区SorLA表达；40只C57BL/6雌性小鼠随机分为假手术+空白病毒（sham+NC-sgRNA）组、卵巢切除+空白病毒（OVX+NC-sgRNA）组、卵巢切除+空白病毒+E2（OVX+NC-sgRNA+E2）组、卵巢切除+SorLA敲降病毒+E2 组（OVX+Sorl1-sgRNA+E2）组，通过高尔基染色检测小鼠海马CA1区神经元树突棘密度；通过Western blotting 和免疫组织化学染色检测小鼠海马CA1区突触后致密蛋白95（PSD-95）和突触蛋白1（SYN1）表达。  结果 与sham组相比，和OVX组和OVX+Fu+E2组SorLA蛋白表达显著降低（P<0.05），和OVX+E2组差异无显著性（P >0.05）； 与sham+NC-sgRNA组相比，OVX+NC-sgRNA组和OVX+Sorl1-sgRNA+E2组树突棘密度和PSD-95、SYN1表达显著降低（P <0.05），与OVX+NC-sgRNA+E2组差异无显著性（P>0.05）。  结论 E2可通过雌激素受体上调SorLA表达，进而增加小鼠海马神经元树突棘密度和突触蛋白表达。

关键词: 雌二醇, 雌激素受体, 分拣蛋白相关受体A, 免疫印迹法, 免疫组织化学, 高尔基染色, 小鼠

Abstract:

Objective To study the effect and mechanism of estradiol (E2) regulating sortilin-related receptor A (SorLA) expression on hippocampal spine density and synaptic protein expression of mouse.   Methods Thirty-two C57BL/6 female mice were randomly divided into sham group, ovariectomized(OVX) group, OVX+E2 group, and OVX+fulvestrant(Fu)+E2 group; The SorLA expression in hippocampal CA1 region of mouse was detected by Western blotting and immunohistochemical staining. Forty C57BL/6 female mice were randomly divided into sham+NC-sgRNA group, OVX+NC-sgRNA group, OVX+NC-sgRNA+E2 group, and OVX+sortilin-related receptor 1(Sorl1)-sgRNA+E2; the spine density in hippocampal CA1 region was detected by Golgi-Cox staining and the postsynaptic density protein 95 (PSD-95) and synapsin-1 (SYN1) expression in hippocampal CA1 region were detected by Western blotting and immunohistochemical staining.   Results Compared with the sham group, the SorLA expression in OVX group and OVX+Fu+E2 group decreased significantly (P<0.05), but OVX+E2group had no significant difference (P>0.05). Compared with the sham+NC-sgRNA group, the density of dendritic spines, and the PSD-95, SYN1 expression of OVX+NC-sgRNA group and OVX+Sorl1-sgRNA+E2 group decreased significantly (P<0.05), but OVX+NC-sgRNA+E2 group had no significant difference (P>0-05).   Conclusion E2 may upregulate SorLA expression through estrogen receptor and then promote the hippocampal spine density and synaptic protein expression of mouse.

Key words: Estradiol, Estrogen receptor, Sortilin-related receptor A, Western blotting, Immunohistochemistry, Golgi-Cox staining, Mouse 

中图分类号: