解剖学报

›› 2009, Vol. 40 ›› Issue (4): 604-608.doi: 10.3969/j.issn.0529-1356.2009.04.017

• 论著 • 上一篇    下一篇

中国人肝癌分泌型卷曲相关蛋白1基因遗传的不稳定性

孙建中1*; 徐丽倩3 ; 李翔2; 郭晓晔1 ; 柴建平1 ;江伟春1 ;郑国荣1; 叶再元2   

  1. 1．浙江省青田市人民医院外科，浙江青田 323900;2.浙江省人民医院，浙江省胃肠病重点实验室，杭州 310014;3浙江大学医学院组织学胚胎学教研室，杭州 310058
  • 收稿日期:2008-11-13 修回日期:2009-02-25 出版日期:2009-08-06
  • 通讯作者: 孙建中

Genetic instability of the sFRP1 gene in hepatocellular carcinoma in Chinese people

  1. 1.Department of Surgery, Zhejiang Qingtian Hospital，Zhejiang Qingtian 323900, China;2.Zhejiang People’s Hospital，Zhejiang Gastroenterology Key Laboratory，Hangzhou 310014,China;3.Department of Histology and Embryology, Zhejiang University School of Medicine, Hangzhou 310058,China
  • Received:2008-11-13 Revised:2009-02-25 Online:2009-08-06
  • Contact: SUN Jian-zhong1

关键词: 肝细胞 肝癌, EM>sFRP1/EM>基因, 微卫星不稳定, 杂合性缺失, 聚合酶链-单链构象多态性

Abstract: Objective To examine loss of heterozygosity (LOH) and microsatellite instability (MSI) of locus D8S532 on chromosome 8 and their influence on the expression of sFRP1 in the hepatocellular carcinoma (HCCs), which may provide an experimental evidence for clarifying the mechanism of EM>sFRP1/EM> gene and tumor development. Methods DNA was extracted from formalin-fixed paraffin-embedded tissues. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and ordinary silver stain were used to study LOH and MSI of locus D8S532. Envision immunohistochemistry, Leica-Qwin computerized imaging system and Image-Pro PluS (IPP) version 4.5 professional imaging analysis software were used to assess the expression of sFRP1. Results The detection rates of LOH and MSI of locus D8S532 in the 36 specimens of HCC were 11.11% and 8.33% respectively. The down-regulation of sFRP1 was observed in 31 of 36 HCCs (86.11%) compared with non-carcinoma liver tissues, and the positive rate of sFRP1 protein of the HCCs was 52.78%( 19/36 ). The frequency of LOH was lower in the cases with positive expression of sFRP1 protein than those negative (0 vs 23.53%, P<0.05). Conclusion It was a common phenomenon that expression of sFRP1 protein is negative or low in Chinese with HCCs. The genetic instability of EM>sFRP1/EM> gene was one of causes, which lead to HCCs. LOH may play a major role in negative expression of sFRP1.

Key words: Hepatocellular carcinoma, EM>sFRP1/EM> gene, Microsatellite instability, Loss of heterozygosity, Polymerase chain reactionsingle strand conformation poly

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